PCR快速检测常见具有毒性质粒的沙门氏菌  

Rapid Detection of Salmonella Virulence-Associated Plasmid Genes by PCR

作　　者：马立农[1] 刘华伟[2] 陈跃进[3] 曾智[3] 

机构地区：[1]深圳职业技术学院,深圳518055 [2]西北农林科技大学,杨凌712100 [3]湖南人文科技学院,娄底417000

出　　处：《世界科技研究与发展》2011年第1期36-38,共3页World Sci-Tech R&D

摘　　要：针对常见具毒性质粒的沙门氏菌的spvR基因,设计出PCR特异引物spvR-P35-P36,以便快速检测出具有毒性质粒的沙门氏菌。验证采用13株沙门氏菌和4株非沙门氏菌,其中6株常见具有毒性质粒的沙门氏菌均获得特异性扩增,7株不含毒性质粒的沙门氏菌均未获得特异性扩增,4株非沙门氏菌检测结果为阴性。结果表明,本文中设计的引物具有高度的特异性,适用于常见具有毒性质粒沙门氏菌的快速检测,此引物已获国家发明专利。For detecting of virulence-associated plasmid genes is very important in assessing virulence for Salmonella, a pair of specific oligonucleotide primers is designed. And a detection method is developed by PCR. Thirteen strains of Salmonella and four strains of other bacterial species are tested by PCR using a pair of oligonucleotide primers spvR-P35-P36. Six toxic Salmonella strains containing spvR genes in virulence-associated plasmid are positively identified. Seven non-toxic Salmonella strains and four non-Salmonella strains are all negative as determined by the PCR method ,which well suits for the detection of toxic Salmonella strains based on the existence of virulence-associated plasmid genes. It is awarded the country invention patent.

关 键 词：沙门氏菌 毒性质粒 基因扩增 特异引物 

分 类 号：Q93-33[生物学—微生物学]