MRP3在胰腺癌及胰腺癌细胞系的表达及意义  

Expression and significance of multidrug resistance-associated protein 3 in pancreatic cancer and pancreatic cancer cell lines

作　　者：高玮[1] 王从俊[1] 尤天庚[1] 卢爱国[1] 过欣来[1] 杨鹞[1] 张辉[1] 

机构地区：[1]上海同济大学附属东方医院普通外科,上海200120

出　　处：《中华内分泌外科杂志》2011年第1期14-17,共4页Chinese Journal of Endocrine Surgery

基　　金：国家自然科学基金资助项目（30872510）;上海市科委科研计划项目（064119620）;上海市自然科学基金（10ZR1424300）

摘　　要：目的探讨多药耐药相关蛋白3（MRP3）在胰腺癌标本及多种肿瘤细胞中的表达及意义。方法利用免疫组织化学的方法检测30例胰腺癌与癌旁组织中MRP3的表达，同时通过RT—PCR检测7种肿瘤细胞及人胚肾293T细胞在mRNA水平的表达情况，采用抗MRP3的单克隆抗体通过流式细胞仪检测MRP3蛋白的表达。结果在各种胰腺癌标本中，MRP3在细胞核中的表达阳性细胞较正常组织明显增多（尸=0．03），表达强度明显增强（P=0．015），而在胞浆上二者表达差异无统计学意义（P=0．472）；MRP3mRNA在3种人胰腺癌细胞中皆有表达，在其他5种细胞无表达，MRP3蛋白在胰腺癌细胞系BxPC-3及AsPC-1中阳性率较高（分别为68．5％和33．6％），而在PANC-1细胞中仅有5．4％表现为MRP3阳性。结论MRP3在胰腺癌中的表达与正常胰腺组织的差别主要在细胞核，而在培养细胞中可能以组织特异性的方式表达不同，BxPC-3细胞系是体外胰腺癌耐药研究的一种选择。Objective To investigate the expression and significance of M RP3 in pancreatic cancer and different tumor cells. Methods MRP3 expression was detected in 30 cases of pancreatic cancer tissues and the adjacent tissues through immunohistochemical method. RT-PCR was used to detect MRP3 mRNA in 7 tumor cell lines and human embryo kidney cell lines 293T. Monoclonal antibody against MRP3 was employed to detect MRP3 protein expression through flow cytometry. Results There was significant difference for MRP3 expression in nucleus between the pancreatic cancer and normal tissues beside the cancer （ P = 0. 003 ） while no significant difference was found in cytoplasm （ P = 0. 472 ）. MRP3 mRNA expression was found in 3 pancreatic carcinoma cell lines. The positive expression rate of MRP3 protein was relatively high in BxPC-3 and AsPC-1 cells （68.5% and 33.6% respectively） while it was only 5.4% in PNAC-1. Conclusions The difference of MRP3 expression in pancreatic cancer and normal tissues lies mainly in nucleus. BxPC-3 may serve as cellular models for in vitro studies on muhidrug resistance of pancreatic carcinoma.

关 键 词：多药耐药相关蛋白-3 胰腺癌 免疫组化 耐药 

分 类 号：R735.9[医药卫生—肿瘤]