反硝化细菌Klebsiella sp.DB-1的分离鉴定与活性研究  

Identification and denitrification activity of an isolated denitrifier

作　　者：刘咏[1] 钱家忠[2] 魏兆军[1] 王璐璐[2] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]合肥工业大学资源与环境工程学院,安徽合肥230009

出　　处：《中国科学技术大学学报》2011年第1期16-21,61,共7页JUSTC

基　　金：国家自然科学基金(40872166;40672154);新世纪优秀人才计划(NCET-06-0541)资助

摘　　要：从巢湖芦苇湿地分离筛选出一株异养反硝化细菌,对其进行鉴定,并进行了反硝化活性研究,目的是为了研究地下水硝酸盐污染的生物修复机理.用酒石酸钾钠培养基从湿地土壤中富集并分离出反硝化细菌,对该菌株进行了16SrDNA鉴定及系统发育分析,并研究了单一碳源、碳氮比对其反硝化活性的影响,以及其对水中硝酸盐氮含量的适应性.分离出了一株异养反硝化细菌,具有较高的反硝化活性,命名为Klebsiellasp.DB-1,革兰氏阴性球形,兼性厌氧,16SrDNA序列分析表明,该菌株与Klebsiella sp.的相似性为99.6%以上;该菌株利用乙酸钠为碳源,采用碳氮比(C/N)为3,水中初始硝酸盐氮含量在100mg/L以内,120h几乎完全去除水中硝酸盐氮.菌株Klebsiellasp.DB-1为异养反硝化细菌,具有较广的碳源谱,能够有效地去除水中硝酸盐氮.In order to research the mechanism of nitrate-contaminated groundwater bioremediation,a heterotrophic denitrifier was isolated and identified from the reed-wetland in Chaohu lake,and its denitrification activity was studied.Denitrifer was isolated on sodium potassium tartrate agar,and 16S rDNA identification and phylogenetic analysis of the strain were performed.The influence of solo carbon source and C/N ratio on the denitrification activity was studied and the suitability of the strain for nitrate content in water was researched.A heterotrophic denitrifier with high denitrification activity was isolated and named DB-1.The isolated strain was gram negative,a facultative anaerobe,and a micrococcus bacterium.The 16S rDNA sequence analysis revealed that strain DB-1 had a similarity of 99.6% to Klebsiella sp.When sodium acetate was used as carbon source,NO-3-N could be removed almost completely from water at 120 h while keeping C/N ratio of 3 and the initial nitrate concentration was 100 mg/L.The strain DB-1 was a heterotrophic denitrifier,with extensive carbon source scope and capable of removing nitrate effectively from water.

关 键 词：异养反硝化细菌 16S rDNA序列鉴定 活性研究 

分 类 号：X172[环境科学与工程—环境科学]