自身抗体在丙型肝炎和自身免疫性肝炎中的特点分析  

The feature of autoantibodies in hepatitis C and autimmune hepatitis

作　　者：王蓉[1] 耿秀蓉[2] 覃沙[1] 洪华[1] 谭太昌[1] 

机构地区：[1]四川省人民医院检验科,四川成都610071 [2]四川省第二中医医院检验科,四川成都610031

出　　处：《四川医学》2011年第2期253-256,共4页Sichuan Medical Journal

摘　　要：目的分析自身抗体在丙型肝炎和自身免疫性肝炎中的特点。方法采用间接免疫荧光法和/或欧蒙印迹法检测97例丙型肝炎患者、31例临床确诊的自身免疫性肝炎患者和100例健康人血清中的ANA、SLA/LP、SAMA、AMA、AMA-M2、LC-l、APCA、LKM-1等自身抗体。结果 HCV组中,抗ANA、LKM-1、LC-1、SAMA、AMA-M2、APCA体阳性率分别是38.14%、2.06%、1.03%、2.06%、1.03%和3.09%,而且阳性滴度都比较低,大部分处于1:100。AIH组中,抗ANA、SLA/LP、抗LKM-l、LC-1、SAMA、AMA-M2、APCA抗体阳性率分别是80.65%、16.13%、12.90%、22.58%、58.06%、12.90%、3.22%,AIH组阳性率还是明显高于HCV组(P<O.05),以高滴度为主,大多数大于等于1:320。丙型肝炎RNA阳性组自身抗体阳性率高于RNA阴性组。结论丙型肝炎患者血清中存在多种自身抗体,但滴度较低;自身免疫性肝炎具有高滴度的自身抗体水平;HCV引起的自身免疫紊乱尤其是病毒处于复制状态是造成机体免疫损伤,加重丙肝病毒血症的极其重要的因素。Objective To analyze the feature autoantibodies in Hepatitis C and Autoimmune Hepatitis.Methods The serum samples from 97 patients with HCV and 31 patients with AIH were examined for autoantibodies such as ANA、SLA/LP、SAMA、AMA、AMA-M2、LC-1、 APCA、LKM-1 by indirect immunefluorescence or immuoblot assay.Results Positive rates of ANA、LKM-1、LC-1、SAMA、AMA-M2、APCA were respectively 38.14%、2.06%、1.03%、2.06%、1.03% and 3.09% in Hepatitis C.Positive rates of ANA、SLA/LP、抗 LKM-1、LC-1、SAMA、AMA-M2、APCA in autoimmune hepatitis were respectively 80.65%、16.13%、12.90%、22.58%、58.06%、12.90%、3.22% and the titer of autoantibodies were low in Hepatitis C group,However,the higher titer of autoantibodies was dominated in AIH.The positive RNA in HCV patients is relvent with autoantibodies.Conclusion There are many autoantibodies in serum of patients with HCV,but the titer is very low.The autoantibodies titer in AIH is high.HCV infection may result in autoimmune disorder,which may be one of important factors which lead to immune damage and aggravate HCV viremia.

关 键 词：丙型肝炎 自身免疫性肝炎 自身抗体 免疫印迹法 间接免疫荧光法 

分 类 号：R575.1[医药卫生—消化系统] R512.63[医药卫生—内科学]