78例消化道类癌中p16和Ki-67的表达  

Expressions of Ki-67 and p16 in 76 Patients with Gastrointestinal Carcinoid

作　　者：徐洪[1] 王箴[1] 曾训 

机构地区：[1]广东省深圳市蛇口人民医院病理科,518067

出　　处：《中华全科医学》2011年第3期350-351,共2页Chinese Journal of General Practice

基　　金：广东省深圳市科技计划项目(200802143)

摘　　要：目的联合检测抑癌基因p16蛋白和肿瘤增殖抗原Ki-67在消化道类癌中的表达,探讨其与分化、侵袭、转移的关系。方法收集消化道类癌石蜡包埋组织78例,其中典型类癌33例,非典型类癌26例,低分化类癌19例。运用免疫组化SP法检测p16和Ki-67的表达情况。结果 p16蛋白在典型类癌、非典型类癌、低分化类癌中表达阳性率分别为87.8(29/33)、34.61%(9/26)、21.53%(4/19),典型类癌与后两者相比差异有统计学意义(P<0.05),p16蛋白表达与类癌的浸润、转移无关。Ki-67增殖指数在典型类癌、非典型类癌、低分化类癌中表达阳性率分别为21.21%(7/33)、61.54%(16/26)、78.94%(15/19),典型类癌与后两者相比差异有统计学意义(P<0.05),且与p16表达结果呈负相关(r=-0.67,P<0.05)。Ki-67增殖指数且与类癌的浸润、转移有关,在早期癌组、进展期癌组的表达阳性率分别为27.45%(14/51)、74.07%(20/27),两者相比差异有统计学意义(P<0.05),在无淋巴结转移组、有淋巴结转移组表达阳性率分别为31.92%(15/47)、83.87%(26/31),两者相比差异有统计学意义(P<0.05)。结论联合测定增殖抗原Ki-67和抑癌基因p16蛋白,可以为类癌分化、侵袭、转移提供较为客观简捷的诊断指标,为临床治疗和预后判断提供客观依据。Objective To detected the levels of Ki-67 and p16 in the tissues of gastrointestinal carcinoid （GC）, and evaluate their relations with the differentiation, invasion, metastasis of tumors. Methods Immunohistoehemical staining（ SP methods） Was used to detect the expression of Ki-67 and p16 in 78 cases of GC（typical GC 33 cases,atypical GC 26 cases,poorly differentiated GC 19 cases）. Results The positive expression rates of p16 protein in typical carcinoid, atypical carcinoid and undifferentiated carcinoid were 87.8 % （ 29/33 ）, 34.61% （ 9/26 ） and 21.53 % （ 4/19 ）, respectively ; the expression level in typical carcinoid was significantly higher as compared to the other two types of carcinoid（ P 〈 0.05 ）. The positive expression rates of p16 protein had no relativity in invasion and proliferation. The positive expression rates of Ki-67 protein in typical carcinoid, atypical carcinoid and undifferentiated carcinoid were 21.21% （7/33） ,61.54% （16/26） ,78.94% （15/19） ,respectively;the expression level in atypi- cal carcinoid and undifferentiated carcinoid was significantly higher as compared to that in typical carcinoid （P 〈 0.05 ）, and the positive expression rates of Ki-67 protein was significant marked in deep infiltration（74.7% ,P 〈 0.05） and lymphoid metastasis （83.87% ,P 〈 0.05）. Conclusion To detect the level of Ki-67 and p16 is helpful to evaluate the differentiation, invasion, metastasis of GC, and judge the prognosis of patients with GC.

关 键 词：消化道类癌 免疫组织化学 肿瘤增殖抗原(Ki-67) 抑癌基因(p16) 

分 类 号：R735[医药卫生—肿瘤] R730.23[医药卫生—临床医学]