一株氯苯降解新菌株的分离鉴定及其降解特性研究  

Isolation,Identification and Biodegradation Characteristics of a Novel Chlorobenzene-degrading Bacterial Strain

作　　者：冷守琴[1] 魏芳[1] 张丽丽[1] 陈建孟[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《环境科学与技术》2011年第2期6-11,共6页Environmental Science & Technology

基　　金：国家高新技术研究发展计划(863)项目(2006AA06A310);浙江省科技计划项目(2008C23082);浙江省科技计划项目(2008C23084)

摘　　要：采集某净化氯苯废气的生物滴滤床填料表面的生物膜,分离纯化后得到1株能高效降解氯苯的菌株L2,基于菌株生理生化特征、16SrDNA序列系统学分析和Biolog鉴定,可确定该菌株为皮氏罗尔斯顿菌(Ralstonia pickettii),其为新发现的具有氯苯降解性能的菌株。该菌株最佳生长温度和pH分别为30℃和7,在最佳生长条件下菌株对220mg/L的氯苯在3d内的去除率可达99%。酵母浸出汁(YE)的添加对菌株的生长和氯苯降解速率有明显的促进作用,添加10、20、50、100、150mg/LYE,菌株的比生长速率从0.14h-1提高到0.23 h-1,氯苯的平均降解速率从3.03mg/h提高到4.40mg/h。基于邻苯二酚双加氧酶活性的测定,初步认为氯苯的生物降解过程中产生了邻苯二酚,邻苯二酚1,2双加氧酶是邻苯二酚开环的关键酶之一。A bacterial strain L2 able to efficiently degrade chlorobenzene was isolated from biotrickling filer treating waste gas containing chlorobenzene.Based on physiological and biochemical characteristics,sequence analysis of 16S rDNA and Biolog identification system,the isolation strain was identified as Ralstonia pickettii which was possible a new species able to degrade chlorobenzene.The optimal conditions for growth of the strain were at 30 ℃ and pH 7.0.Under the optimal growth conditions,the removal efficiency up to 99% for 220 mg/L of chlorobenzene was obtained within 3 days.Yeast extract（YE）could remarkably facilitate the growth of L2 and chlorobenzene degradation.Adding YE of 10,20,50,100 and 150 mg/L,the specific growth rate of L2 would increase from 0.14 h^-1 to 0.23 h^-1,while average degradation rate of chlorobenzene by L2 was correspondingly increased from 3.03 mg/h to 4.40 mg/h.Based on preliminary determination of catechol dioxygenase activity,it was proposed that chlorobenzene was converted to pyrocatechol during the biodegradation,and ring cleavage of pyrocatechol was primarily via catechol 1,2-dioxygenase.

关 键 词：氯苯 生物降解 皮氏罗尔斯顿菌 邻苯二酚双加氧酶 

分 类 号：X172[环境科学与工程—环境科学]