油菜花粉脂溶性提取物对前列腺癌细胞生长抑制的实验研究  

Suppression Effect of Brassica Campestris Pollen Extract on the Growth of Prostate Cancer Cell in vitro

作　　者：朱赤红[1] 杜灵彬[1] 高赟[1] 钱丽娟[1] 顾琳慧[1] 曹明富[2] 

机构地区：[1]浙江省肿瘤医院肿瘤研究所,杭州310222 [2]杭州师范大学生命与环境科学学院

出　　处：《浙江中西医结合杂志》2011年第2期84-86,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省科技攻关计划项目(No.2005C33005)

摘　　要：目的:研究油菜花粉脂溶性提取物(BPE)对体外培养的人前列腺癌细胞的杀伤效应。方法:以人前列腺癌细胞PC-3和DU-145为研究对象,光镜观察用药前后细胞形态,透射电镜观察细胞凋亡的形态变化;CCK-8法测定油菜花粉脂溶性提取物对细胞生长抑制率,流式细胞术检测BPE对PC-3和DU-145细胞周期的影响。结果:不同浓度油菜花粉脂溶性提取物(BPE)对两株前列腺癌细胞有明显的抑制作用;形态学观察BPE处理后的前列腺癌细胞出现显著改变;流式细胞仪检测结果显示:PC-3及DU-145细胞在油菜花粉脂溶性提取物处理48h后,细胞周期出现变化,G0/G1期比例上升,S期和G2-M期比例下降。结论:油菜花粉脂溶性提取物对两株前列腺癌细胞的生长有明显的抑制作用。Objective：To study the effects of Brassica campestris pollen extract（BPE）on prostate cancer cell line in vitro.Methods：PC-3 and DU-145 cell line from prostate cancer were treated with various concentrations of BPE.Morphologic change was observed with light microscope and apoptosis change by electron microscope.The suppression rate of the growth of prostate cancer cell was evaluated by using CCK-8 assay.Changes in prostate cancer cell cycle were observed by FAC SCailibur.Result：The growth of prostate cancer cells were markedly inhibited after being treated with BPE.After the treatment,obvious morphological change was observed;FCM showed that the rate of G0/G1 phase was increased and that the rate of S and G2-M phase was decreased in the cell cycle.Conclusion：BPE may have remarkable effect on inhibiting the growth of PC-3 and DU-145 cell line from prostate cancer.

关 键 词：油菜花粉脂溶性提取物 人前列腺癌细胞 生长抑制 肿瘤 

分 类 号：R737.25[医药卫生—肿瘤]