淡豆豉中异黄酮提取工艺的优化研究  

Research on Optimization of Isoflavone Extracting Technique from Semen Sojae Praeparatum

作　　者：牛丽颖[1] 蔡广华[1] 刘敏彦 王鑫国[1] 

机构地区：[1]河北医科大学中医学院,河北石家庄050091 [2]河北以岭医药研究院有限公司,河北石家庄050035

出　　处：《大豆科学》2011年第1期164-167,共4页Soybean Science

基　　金：河北省自然科学基金资助项目(C2009001061)

摘　　要：采用单因素和正交试验相结合的方法,以淡豆豉总异黄酮和染料木素的提取量为评价指标,优选淡豆豉中异黄酮的提取工艺。结果表明淡豆豉中提取异黄酮的最佳工艺为:淡豆豉药材8倍量70%乙醇回流提取3次,每次2 h。The best extracting technique of Semen Sojae Paratum（SSP） were optimized by orthogonal test.The extracting contents of total isoflavone and genistein in SSP were determined by UV detector and HPLC,respectively.The results showed that the best technological process of isoflavone extraction was to reflux SSP by 70% ethanol 3 times and 2 hours for each time.The content of 70% ethanol was 8 times of SSP.

关 键 词：淡豆豉 异黄酮 染料木素 UV HPLC 

分 类 号：TQ461[化学工程—制药化工]