玻璃化超低温保存对怀菊花再生苗形态及生理的影响  

Effect of Cryopreservation by Vitrification on Morphology and Physiology of Huaiqing Chrysanthemum(Dendranthema morifolium) Regeneration Plantlets

作　　者：赵喜亭[1,2] 宋萍萍[1] 王苗[1] 赵月丽[1] 郭婧[1] 李明军[1,2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,河南新乡453007

出　　处：《湖北农业科学》2011年第3期533-535,543,共4页Hubei Agricultural Sciences

基　　金：河南省科技攻关计划重点项目(0623030700)

摘　　要：以怀菊花中的优良品种小黄菊为试材,研究了玻璃化超低温保存对怀菊花再生苗形态及生理指标的影响。结果表明,与常温苗相比,继代培养的玻璃化超低温保存再生苗前期(0～15 d)生长较慢,中期(15～30 d)开始快速生长,至45 d时,二者长势基本一致;叶片的可溶性糖、可溶性蛋白质和叶绿素含量均没有显著的差异。玻璃化超低温保存能够保持其形态和生理的稳定性。In this paper,Xiaohuangju,a superior variety of Huaiqing chrysanthemum（Dendranthema morifolium）,was taken as materials,to study the effects of cryopreservation by vitrification on morphology and physiology of Huaiqing Chrysanthemum regeneration plantlets.The results indicated that comparing with the plantlets preserved at room temperature,the plantlets after cryopreservation by vitrification grew slowly in early phase（0～15 d）;and then began to grow faster in medium term（15～30 d）;till the 45th day,they seemed congruously in growth.There were no significant difference between the plantlets after cryopreservation by vitrification and the plantlets preserved at room temperature on the average increasing value of internodal length,plant height,leaf number,and the contents of soluble sugar,soluble protein and chlorophyll.Thus morphological and some physiological stability could be maintained via vitrification in Huaiqing chrysanthemum.

关 键 词：怀菊花 超低温保存 再生苗 形态 生理指标 

分 类 号：S325.12[农业科学—作物遗传育种] S682.11[农业科学—农艺学]