APE1单核苷酸多态性与肺癌易感性关系的研究  

Association of single nucleotide polymorphism of APE1 gene with susceptibility to lung cancer

作　　者：李峥[1] 李梦侠[1] 廖玲[1] 王东[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆400042

出　　处：《重庆医学》2011年第6期528-531,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30670628)

摘　　要：目的探讨中国重庆汉族人群脱嘌呤/脱嘧啶核酸内切酶1(APE1)基因单核苷酸多态性与肺癌易感性的关系。方法采用病例对照研究,应用相对的两对引物-聚合酶链反应(PCR-CTPP)技术检测455例肺癌患者和443例健康人APE1-141T/G和APE1 148Asp/Glu单核苷酸多态性(SNP)。结果 APE1-141G/G基因型相对于T/T基因型,显著减少了患肺癌的风险(OR=0.62;95%CI为0.42～0.91);单倍体分析中,APE1-141T/148Glu单倍体相对于-141T/148Asp单倍体,显著增加了患肺癌的风险(OR=1.28,95%CI为1.01～1.62)。结论 APE1基因与中国重庆汉族人群肺癌易感性密切相关,APE1-141T/148Glu单倍体可能是肺癌的重要遗传易感因素。Objective To explore the genetic association between the single nucleotide polymorphism（SNP） of APE1 gene and the susceptibility to lung cancer among Chinese Han people in Chongqing.Methods A population based case control study was conducted in 443 healthy controls and 445 lung cancer patients.The SNP of APE1―141T/G and APE1 148Asp/Glu were detected by polymerase chain reaction with confronting two-pair primers（PCR-CTPP）.Results For the APE1 ―141T/G polymorphism,individuals with G/G genotype significantly decreased the risk of developing lung cancer compared with those harboring T/T genotype（OR=0.62;95%CI,0.42―0.91）and APE1 ―141T/148Glu haplotype markedly increased the risk,compared to ―141T/148Asp haplotype（OR=1.28;95%CI：1.01―1.62）,according to haplotype analysis.Conclusion This study confirms the close relationship between APE1 gene and Lung cancer.APE1 ―141T/148Glu haplotype may serves as an important genetic susceptibility factor for lung cancer.

关 键 词：肺肿瘤 脱嘌呤/脱嘧啶核酸内切酶1 单核苷酸多态性 

分 类 号：R734.2[医药卫生—肿瘤]