桂花中总黄酮提取工艺及其抗氧化活性的研究  

Study on extraction of the total flavonoids in Osmanthus fragrans Lour. and its antioxidative activity

作　　者：郭娇娇[1] 罗佳[1] 宫智勇[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,武汉430023

出　　处：《武汉工业学院学报》2011年第1期5-8,共4页Journal of Wuhan Polytechnic University

摘　　要：利用乙醇回流法提取桂花中总黄酮类物质,并通过单因素实验以及正交实验确定桂花中总黄酮的最佳提取工艺条件。实验结果表明,最佳提取工艺条件为乙醇浓度70%,提取温度50℃,料液比1∶50(W∶V),提取时间2h。采用DPPH法测定了桂花总黄酮提取液清除DPPH.自由基的能力,结果显示,在最佳提取工艺条件下黄酮提取液对DPPH.自由基的清除率达到96.8%,有较强的抗氧化活性。By means of monothetic and orthogonal test,the optimum extraction conditions of total flavonoids with ethanol refluxing in Osmanthus fragrans Lour.were determined.The optimum conditions of Osmanthus fragrans Lour.were obtained as follows： 70 % ethanol as extraction solvent at 50 ℃ for 2h,and the ratio of material to solvent was 1∶50（W∶V）.DPPH was used to measure and compare the capacity of scavenging DPPH free radicals of Osmanthus fragrans Lour.,the ability of scavenging the DPPH free radicals of the Osmanthus fragrans Lour.sample was up to 96.8 % under the optimum conditions,it showed strong antioxidant activity.

关 键 词：桂花 总黄酮 提取 抗氧化 

分 类 号：S38[农业科学—农艺学] TS201.2[轻工技术与工程—食品科学]