中药二步序贯治疗对哮喘小鼠气道组织核因子κB系统的影响  

The effects of two-step sequential therapy on signal pathway of NF-κB on airway inflammation in asthmatic mice

作　　者：杨召川[1] 陈蕾[2] 刘秀美[2] 常红[2] 曲政海[2] 

机构地区：[1]青岛大学医学院研究生部,山东青岛266003 [2]青岛大学医学院附属医院儿科

出　　处：《中国中西医结合儿科学》2011年第1期23-26,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：山东省自然科学基金资助课题(Y2007C113)

摘　　要：目的探讨中药二步序贯治疗对哮喘小鼠气道组织核因子κB(NF-κB))系统的影响。方法健康雌性昆明系小鼠40只随机分为正常对照组、模型对照组、布地奈德组和二步序贯组,每组10只。采用卵清白蛋白致敏建立哮喘小鼠模型,并分别予以布地奈德和二步序贯干预治疗。观察哮喘小鼠气道组织病理变化,气道组织NF-κB、GATA结合蛋白3(GATA-3)以及肺泡灌洗液白细胞介素4(IL-4)的变化。结果布地奈德组和二步序贯组气道组织炎性改变较模型对照组明显减轻,较正常对照组明显加重,差异均有统计学意义(P<0.01);布地奈德组和二步序贯组气道炎症改变差异无统计学意义(P>0.05)。布地奈德组和二步序贯组气道组织NF-κB、GATA-3、IL-4表达水平较模型对照组均降低,较正常对照组均升高,差异均有统计学意义(P<0.01);布地奈德组和二步序贯组比较,差异无统计学意义(P>0.05)。结论二步序贯治疗可能通过抑制NF-κB信号转导通路活性,进而降低GATA-3的表达,降低Th2类细胞因子,减轻哮喘小鼠气道炎症。Objective To study the effects of two-step sequential therapy on signal pathway of NF-κB of airway inflammation in asthmatic mice.Methods Forty healthy female Kunming mice were divided into four groups randomly（n=10,in each group）：normal control group,asthma group,budesonide group and two-step sequential therapy group.The asthmatic models were established by ovalbumin,and budesonide and two-step sequential therapy were applied to them.Then the pathologic changes in airway tissues and bronchoalveolar lavage fluid（BALF）in cytology were observed.The level of nuclear factor-κB（NF-κB）and GATA-3 in airway tissues was detected by immunohistochemical method.The level of interleukin-4（IL-4）in BALF was detected by enzyme-linked immunosorbent assay（ELISA）.Results The treatment groups had less inflammatory cell infiltration than asthmatic group（P0.01）,and more than normal control group（P0.01）.There was no significant difference between the budesonide group and two-step sequential therapy group（P0.05）.The level of NF-κB,GATA-3 and IL-4 decreased in the budesonide group and two-step sequential therapy group compared with the asthmatic group（P0.01）,but increased compared with the normal control group（P0.01）.There was no significant difference between budesonide group and two-step sequential therapy group（P0.05）.Conclusions The two-step sequential therapy may inhibit the activity of the signal transduction pathway of NF-κB,which can reduce the expression of GATA-3 and class cytokines of Th2,hence plays an important role in treating asthmatic mice.

关 键 词：哮喘 人参五味子汤 小青龙汤 核因子ΚB GATA-3 白细胞介素4 小鼠 动物 实验 

分 类 号：R-33[医药卫生]