雌激素受体β1对乳腺癌MDA-MB-231细胞凋亡的影响  

Estrogen receptor β1 induces apoptosis of MDA-MB-231 breast cancer cells by regulating the expression of Bax gene

作　　者：陈莉[1] 杨新华[1] 范林军[1] 张毅[1] 陈显春[1] 任林[1] 姜军[1] 

机构地区：[1]第三军医大学附属西南医院乳腺疾病中心,重庆400038

出　　处：《中华乳腺病杂志（电子版）》2011年第1期25-28,共4页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：国家自然科学基金资助项目(81072157)

摘　　要：目的观察雌激素受体β1(ERβ1)被阻断后对Bax和Bcl-2表达的影响,探讨ERβ1在乳腺癌中的生物学作用机制。方法应用脂质体法,将针对人ERβ1基因的siRNA转染人类乳腺癌细胞株MDA-MB-231。分别用Realtime-PCR、WesternBlot检测ERβ1被干扰前后细胞中ERβ1、Bcl-2、Bax等基因mRNA和蛋白表达水平的变化;通过细胞增殖曲线观察ERβ1基因被干扰后细胞增殖活性的变化;采用流式细胞仪分析细胞凋亡率的改变。计量资料的比较采用t检验或单因素方差分析。结果 RNA干扰技术阻断乳腺癌MDA-MB-231细胞ERβ1基因的表达后,ERβ1mRNA和蛋白水平显著下降(P<0.05),生长曲线显示细胞增殖能力明显增强(P<0.05);pSilencer-ERβ1转染组细胞的Bax基因mRNA及蛋白水平显著下降(P<0.01),Bcl-2基因的表达未发生变化,Bcl-2/Bax比值明显上升;pSilencer-ERβ1转染组细胞凋亡率较未转染组明显减少(t=6.22,P=0.00)。结论 ERβ1可通过调控细胞凋亡相关基因Bax而促进细胞的凋亡,是ERβ1抑制细胞增殖的原因之一。Objective To observe the changes in Bax and Bcl-2 expressions and to investigate the biology role of estrogen receptor （ER） β1 in MDA-MB-231 breast cancer cells by using RNA interference to interrupt ER^I expression. Methods pSilencer-ERβ1 was transfected into MDA-MB 231 breast cancer cells by using cationic liposome Lipofectamine 2000^TM as tranfecting agent. After transfection, the expression levels of mRNA and protein of ERβI , Bcl-2 and Bax were evaluated by real time polymerase chain reaction （RT-PCR） and Western blot, respectively. Cell apoptosis was detected using flow cytometry. Cell growth curve was performed to show the changes in proliferation ability. Comparison between quantitative data was performed sing t test and one factor analysis of variance. Results After ERβ1 siliencing, both mRNA and protein level of ERβ1 and Bax gene decreased significantly compared with the control groups （P〈0. 05）, and cell growth curve showed a marked enhancement in cell proliferation （P〈0.05）. In the pSilencer-ERβ1 transfeetion group, there was an obvious decrease in mRNA and protein level of Bax gene （P〈0.01）; no significant change in the expression of Bcl 2; and the ratio of Bcl-2/Bax increased. The results from flow cytometry showed that cell apoptosis decreased markedly in the pSilencer-ERβ1 transfection group compared with the controls （t = 6. 22, P = 0. 00）. Conclusion ERβI could induce apoptosis of MDA-MB-231 breast cancer cells by regulating Bax gene expression, which may play an important role in ERβ1 inhibiting the growth of MDA-MB-231 breast cancer cells.

关 键 词：乳腺肿瘤 RNA干扰 雌激素受体β1 

分 类 号：R737.9[医药卫生—肿瘤]