金属硫蛋白对γ射线和丝裂霉素C致遗传损伤的拮抗作用  

Antagonistic Effects of Metallothionein on Genetic Damage Caused by γ-Rays and Mitomycin C

作　　者：薛宏伟[1] 郝福英[2] 魏雪涛[1] 文小青 蒋睿 林慰慈[1] 

机构地区：[1]北京医科大学卫生毒理教研室,100083 [2]北京大学生命科学学院

出　　处：《中华预防医学杂志》1999年第5期301-303,共3页Chinese Journal of Preventive Medicine

基　　金：教育部留学回国人员科研启动基金!1994-99

摘　　要：目的探讨兔肝锌金属硫蛋白（Zn－MT）对γ射线和丝裂霉素C（MMC）所致遗传损伤的桔抗作用。方法应用体内外微核试验和单细胞凝胶电泳法分别检测γ射线照射或MMC处理前后给以Zn-MT对微核形成或DNA链断裂的影响。结果664μg／kg剂量Zn-MT对5Gyγ射线诱发的小鼠骨髓微核形成有抑制作用。10、50μg／mlZn-MT能持抗1和3Gyγ射线及0．3μg／mlMMC诱发的g12细胞双核微核细胞率升高，50μg／mlZn-MT能使1Gyγ射线诱发的链断裂减少。结论兔肝ZnMT对γ射线和MMC所致遗传损伤有一定拮抗作用。To study the anhoshc effects of metallothionein(Zn-MT) on genetic damagecaused by γ-rays and mitomycin C (MMC) in the liver of rabbits. methods micronucleus test and DNA strandbreaks were determined in vitro and in vivo with single-cell gel electrophoresis assay to observe the effects of Zn-MToo the microuncleus formation and cleavage of DNA chain before and after γ-rays radiation and MMC treatment.Results A dose of 664μg／kg of 664μg／kg of Zn-MT could inhibit micronucleus formation in the bone marrow of the mice induced by 5 Gy γ-rays radiation(P<0.05). Concentrations of 10 and 50μg／ml Zn-MT could antagonize the increase ub bunicronucleus frequency in g12 cells induced by 1 and 3 Gy γ-rays radiation and 0.3μg／mlment(P<0.05 or P<0.01). A dose of 500μg／ml Zn-MT could reduce the DNA single strand breaks caused by1 Gy γ-rays radiation, but without statistical significance (P>0.05). Conclusion Zn-MT could antagonize thegencetic damage to certain extent in the rabbits caused by γ-rays radiation and MMC treatment.

关 键 词：金属硫蛋白 Γ射线 丝裂霉素C DNA损伤 

分 类 号：R361[医药卫生—病理学]