维生素C和紫杉醇联合作用对乳腺癌细胞MCF-7增殖、细胞周期和凋亡的影响  

EFFECT OF VITAMIN C IN COMBINATION WITH PACLITAXEL ON GROWTH, CELL CYCLE AND APOPTOSIS OF BREAST CANCER MCF-7 CELLS

作　　者：李新莉[1] 何栋成[1] 黄苏君[1] 朱圣陶[1] 付春玲[1] 秦立强[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院营养与食品卫生教研室,苏州215123

出　　处：《营养学报》2011年第1期40-43,48,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81001185)

摘　　要：目的研究维生素C(VC)与紫杉醇联合作用,对人乳癌MCF-7细胞增殖、细胞周期及凋亡的影响。方法 MTT法检测细胞增殖,激光共聚焦观察自噬,流式细胞术测定细胞周期分布和凋亡率变化。结果 VC单独作用,可促进MCF-7的增殖;紫杉醇单独作用及与VC联合作用均可抑制MCF-7的生长,并将细胞阻滞在G2期及诱导细胞凋亡;紫杉醇与VC联合作用对细胞增殖的影响,与二者的用药顺序有关,紫杉醇作用24后联合应用VC,可协同抑制MCF-7的增殖,并且,紫杉醇的IC50最低,即MCF-7对紫杉醇的敏感性最高。结论紫杉醇与VC联合作用对人乳腺癌MCF-7细胞增殖的影响,与二者的用药顺序有关,其作用的机制与影响细胞周期分布和诱导细胞凋亡有关。Objective To study the effect of vitamin C（VC） in combination with paclitaxel on growth,cell cycle and apoptosis in human breast cancer MCF-7 cells.Method Cell proliferation was evaluated by MTT assay and cell autophagy was observed by laser confocal microscopy.Cell cycle distribution and apoptosis were determined by flow-cytometry.Results The growth and proliferation of MCF-7 could be promoted by VC alone,and inhibited by paclifaxel alone or combined with VC to be arrested in G2 phase and apoptotic.The inhibitory effect on proliferation was different due to different orders between the use of paclitaxel and VC.In treatment of paclitaxel with combination of VC,the inhibitory effect on proliferation was markedly enhanced.Under this condition,the IC50 of paclitaxel was the lowest.Conclusions Vitamin C can increase the sensitivity of MCF-7 to paclitaxel through affecting distribution of cell cycle and promoting apoptosis.

关 键 词：VC 紫杉醇 乳癌细胞 化疗敏感性 

分 类 号：R737.9[医药卫生—肿瘤]