MicroRNA下调SiHa中CD147的表达及对其肿瘤生物学活性的影响  

Inhibition of the expression of CD147 by MicroRNA and influence on the biological activities of tumor in SiHa

作　　者：龚宝兰[1] 陈永利[1] 王任晓[1] 段迎春[1] 胡平[1] 

机构地区：[1]郧阳医学院附属人民医院妇产科,湖北十堰442000

出　　处：《南京医科大学学报（自然科学版）》2011年第2期193-198,共6页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨下调宫颈癌细胞系SiHa中CD147蛋白表达对肿瘤细胞增殖成瘤性等生物学活性的影响。方法:构建重组质粒pSilencer 4.1-CMV neo/CD147 siRNA1、2,稳定转染至宫颈癌细胞系SiHa,采用半定量RT-PCR法、Western blot法检测干扰后细胞CD147及凋亡相关基因Bcl-2、caspase-3 mRNA及蛋白的表达;运用MTT法检测干扰后肿瘤细胞增殖变化;裸鼠成瘤实验分析肿瘤细胞CD147蛋白表达下调后的成瘤性。结果:与对照组相比,转染了pSilencer 4.1-CMV neo/CD147 SiRNA 1、2的SiHa中CD147及Bcl-2的mRNA、蛋白表达水平有显著下调,活性caspase-3表达增加,肿瘤增殖活性下降,裸鼠成瘤实验显示干扰后肿瘤细胞成瘤性及癌肿大小均低于对照组。结论:SiRNA转染能有效抑制宫颈癌细胞系SiHa中CD147的表达,且能抑制肿瘤细胞增殖能力,肿瘤的成瘤能力亦明显下降。Objective： To investigate biological activities after CD147 was down-regulated by microRNA in cervical cancer cell line SiHa.Methods：The plasmids pSilencer 4.1-CMV neo/CD147 siRNA was reconstructed,and then they were stablely transfected into SiHa.The expression of CD147,Bcl-2 and caspase-3 were detected by RT-PCR and Western blot assay.Afterwards,the method of MTT was used to analyze the tumor proliferation.The experiment of nude mice was performed to observe the tumor growth.Results：The expression of CD147 mRNA and protein could be inhibited by pSilencer 4.1-CMV neo/CD147 siRNA Sequence 1 and 2.Compared with the control group,cancer cells have more apoptosis and lower proliferation,After cancer cells were transplanted into nude mice,the tumor volume of siRNA groups were smaller than that of the control.Conclusion：The expression of CD147 could be down-regulated effectively by CD147 SiRNA plasmids,the proliferation and the ability of forming tumor could be inhibited evidently after this treatment in cervical carcinoma cells SiHa.

关 键 词：RNA干扰 CD147 SIHA 

分 类 号：R730.231[医药卫生—肿瘤]