细胞角蛋白19 mRNA在非小细胞肺癌外周血中的表达和临床意义  

Clinical significance of cytokeratin 19 mRNA in the peripheral blood of non-small cell lung cancer

作　　者：余辉[1] 黄秀英[1] 王欣[1] 王军业[1] 鹿彬[1] 杨寒[1] 张兰军[1] 傅剑华[1] 

机构地区：[1]华南肿瘤学国家重点试验室中山大学肿瘤防治中心胸科,广州510060

出　　处：《中国医师进修杂志》2011年第1期20-22,共3页Chinese Journal of Postgraduates of Medicine

基　　金：基金项目：广东省医学科学技术研究基金（A2009217）

摘　　要：目的 应用实时荧光定量RT-PCR方法检测肺癌外周血中细胞角蛋白19（CK19）mRNA的表达情况,并探讨其表达的临床意义.方法 应用实时荧光定量RT-PCR方法检测98例肺癌患者（肺癌组）外周血CK19 mRNA的表达情况,并与28例肺良性疾病患者（肺良性疾病组）和20例健康献血者（对照组）比较.结果 肺癌组患者CK19 mRNA阳性率为77.55%（76/98）,对照组为0（0/20）,肺良性疾病组为3.57%（1/28）,三组CK19 mRNA阳性率比较差异有统计学意义（x^2=73.680,P＜0.01）.肺癌组腺癌患者CK19 mRNA阳性率与鳞癌患者比较差异无统计学意义（x^2=0.414,P＞0.05）,淋巴结转移阳性患者CK19 mRNA阳性率与阴性患者比较差异有统计学意义（x^2=17.523,P＜0.01）,Ⅰ期、Ⅱ期和Ⅲ期患者CK19 mRNA阳性率比较差异有统计学意义（x^2=13.453,P＜0.01）.结论 肺癌患者外周血中CK19 mRNA表达较高可能与肺癌转移有关,检测CK19mRNA对于早期判断肺癌转移和复发有一定的临床意义.Objective To evaluate the clinical significance of cytokeratin19（CK19） mRNA in the peripheral blood of patients with non-small cell lung cancer （NSCLC）. Methods The expression of CK19 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR） in the peripheral blood from 98 NSCLC patients （NSCLC group）, 20 benign pulmonary lesion patients （benign pulmonary lesion group） and 20 healthy adults（control group）. Results The positive rate of CK19 mRNA in peripheral blood of NSCLC group, benign pulmonary lesion group and control group was 77.55 % （76/98）, 3.57% （1/28）,0 （0/20） respectively,and there was significant difference among three groups （ x^2 = 73.680,P 〈 0.01 ）. The positive rate of CK19 mRNA in different pathological types of NSCLC had no significant difference （x^2 =0.414, P 〉 0.05 ）, but had significant difference in different lymphy node metastasis status（ x^2 = 17.523, P 〈0.01 ） and different clinical stage （ x^2 =13.453,P 〈 0.01 ）. Conclusions The higher expression of CK19mRNA in peripheral blood of NSCLC may relate to cancer metastasis. Detection of CK19 mRNA has significance in early prediction of its prognosis.

关 键 词：角蛋白质类 癌 非小细胞肺 反转录聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]