PPV感染PK-15细胞后对4种抗病毒细胞因子mRNA转录时相的影响  

Transcriptional profiles of four kinds of antivirus cytokines mRNA on PK-15 cell cultures following infection with Porcine parvovirus

作　　者：耿静微[1] 李厚伟[2] 尹海燕[3] 陈红英[2] 李金磊[1] 韩志涛[1] 崔保安[1] 魏战勇[1] 

机构地区：[1]河南省动物性食品安全重点实验室,河南郑州450002 [2]河南农业大学牧医工程学院,河南郑州450002 [3]河南省农业科学院农业质量标准与检测技术研究中心,河南郑州450002

出　　处：《西北农林科技大学学报（自然科学版）》2011年第2期17-22,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省杰出人才创新基金项目(0621002100)

摘　　要：【目的】了解猪细小病毒(PPV)感染对抗病毒相关细胞因子mRNA转录时相的影响,探讨宿主-病毒之间的作用关系。【方法】对PPV感染PK-15细胞的病变进行了显微观察,运用荧光定量PCR技术,测定和分析PPV感染PK-15细胞后,细胞病毒DNA含量及巨噬细胞炎症蛋白1α(MIP-1α)、转化生长因子β1(TGF-β1)、磷酸甘油酸激酶1(PGK1)和MURR1等细胞因子mRNA相对表达量的变化。【结果】PPV可以快速诱导PK-15细胞产生细胞病变。感染后1 h即可检测到PPV DNA,随着时间的延长,PPV DNA含量逐渐增加,并于48 h时达到峰值,相对含量为1 800左右。MIP-1α和PGK1 mRNA的相对表达量在感染后2 h显著增加,之后下降;TGF-β1 mRNA的相对表达量在72 h时显著增加;MURR1 mRNA的相对表达量在24 h时显著增加,48 h后下降。【结论】PPV感染可引起PK-15细胞抗病毒因子mRNA表达水平升高。【Objective】 The study was done to survey the transcriptional profiles of cytokines,particularly antivirus cytokines mRNA transcriptional profiles of Porcine parvovirus infection,and the host-PPV interaction.【Method】 We used PPV to infect the Porcine Kidney-15 cells,observed the PK-15 cells of inoculation PPV by microscope,measured and analyzed the viral DNA using real-time PCR.We also detected the transcript levels of cytokines MIP-1α,TGF-β1,PGK1 and MURR1 by real-time PCR.【Result】 PPV can induce cytopathic effect in PK-15 cells.The cells can be detected with PPV on 1 h postinoculation（p.i.）,and the relative content of PPV DNA achieved 1 800 on 48 h p.i..We found that the transcript levels of MIP-1α and PGK1 increased obviously on 2 h p.i.,then decreased,the transcript level of TGF-β1 increased obviously on 72 h p.i.,the transcript level of MURR1 increased obviously on 24 h p.i.,then decreased after 48 h p.i..【Conclusion】 The levels of antiviral cytokines increased when PPV infected PK-15 cell.

关 键 词：猪细小病毒 细胞因子 PK-15细胞 转录时相 

分 类 号：S858.28[农业科学—临床兽医学] Q78[农业科学—兽医学]