胰岛素毫微球肺部给药对正常大鼠的降血糖作用  

Hypoglycemic effects of insulin loaded nanoparticles by pulmonary delivery to normal rats

作　　者：沈赞聪[1] 张强[1] 魏树礼[1] 

机构地区：[1]北京医科大学药剂研究室,北京100083

出　　处：《北京医科大学学报》1999年第5期436-438,共3页Journal of Peking University(Health Sciences)

基　　金：天然药物与仿生药物国家重点实验室资助

摘　　要：目的：研究胰岛素毫微球（ｉｎｓｕｌｉｎｌｏａｄｅｄ ｎａｎｏｐａｒｔｉｃｌｅｓ，ＩＮＳＮＰ） 经大鼠肺部给药后的降血糖作用。方法：建立大鼠肺部给药的动物模型，通过葡萄糖氧化酶法测定血糖质量浓度来评价ＩＮＳＮＰ 经肺部给药后对大鼠的降血糖作用。结果：大鼠肺部分别给与５、１０ 、２０ ｕ·ｋｇ－ １ 的ＩＮＳＮＰ，以血糖下降至给药前的７０ ％ 以下所持续的时间（ｄｕｒａｔｉｏｎ ｂｅｌｏｗ ７０ ％ ｌｅｖｅｌ，ＤＢＬ７０％）为考察指标，５ｕ·ｋｇ－ １ 的ＩＮＳＮＰ的ＤＢＬ７０％ 与其对照溶液相近，而１０、２０ ｕ·ｋｇ－１的ＩＮＳＮＰ的ＤＢＬ７０ ％ 均明显大于各自的对照溶液。ＩＮＳＮＰ肺部给药的相对药理生物利用度达到５９．２％ 。结论：与胰岛素溶液相比，ＩＮＳＮＰ经大鼠肺部给药后能显著延长其血糖下降的时间。Objective:To investigate the hypoglycemic effects of insulin loaded nanoparticles (INS NP) after pulmonary delivery to rats. Methods:The pulmonary delivery model was established in normal rats. Blood glucose levels were measured using glucose oxidase method (GOD) to evaluate the hypoglycemic effects .Results:After 5, 10, and 20 u·kg -1 of INS NP were administered from the lung,The duration below 70% level (DBL 70% ) of 5 u·kg -1 INS NP was approximately the same as that of its control solution,while 10 u·kg -1 and 20 u·kg -1 INS NP had greater DBL 70% values than their control (INS SOL) .The relative pharmacological bioavailability of INS NP reached 59.2%. Conclusion: Pulmonary delivery of INS NP to rats can significantly prolong the duration of hypoglycemic effect.

关 键 词：胰岛素 剂量 微球体 肺部给药 降血糖作用 

分 类 号：R977.15[医药卫生—药品]