几种常见桔园螨类Wolbachia基因检测与序列分析  

Gene identification and sequence analysis of Wolbachia in several common mites in citrus orchards

作　　者：邹志文[1] 陈芬[1] 余丽萍[1] 夏斌[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330031

出　　处：《生态学杂志》2011年第3期547-551,共5页Chinese Journal of Ecology

基　　金：江西省自然科学基金项目(0630147)资助

摘　　要：应用共生菌沃尔巴克氏体Wolbachia的wsp、ftsZ和16SrDNA基因特异引物,通过PCR扩增法对采自江西省南昌、九江和赣州等3地桔园内的东方钝绥螨、江原钝绥螨、尼氏真绥螨、巴氏新小绥螨、桔全爪螨和酢浆草如叶螨等6种常见螨类进行了检测。结果表明,在采自南昌桔园内的酢浆草如叶螨体内检测到Wolbachia感染,其wsp、ftsZ和16SrDNA基因长度为593、758和896bp,Genbank登录号依次为GQ162482、GQ162483和GQ162484,而其他5种螨体内均未检测出Wolbachia的感染;同时通过对酢浆草如叶螨体内感染的Wolbachia的wsp基因序列进行系统发育分析,得出其与B大组的Epo组的Wolbachia株十分相近,可能是相近或相同的株。Polymerase chain reaction（PCR）was adopted to examine the wsp,ftsZ,and 16S rDNA of Wolbachia in Amblyseius orientalis,A.eharai,Euseius nicholsi,Neoseiulus barkeri,Panonychus citri,and Tetranychus harti from the citrus orchards in Nanchang,Jiujiang,and Ganzhou of Jiangxi Province.It was found that only T.harti from the citrus orchards in Nanchang was infected by Wolbachia,with the fragments length of Wolbachia wsp（Accession No.GQ162482）,ftsZ（GQ162483）,and 16S rDNA（GQ162484）being 593,758,and 896 bp,respectively,while the other five species were uninfected.The Wolbachia in T.harti was very similar to the group Epo in subgroup B,based on the phylogenetic analysis of wsp gene.

关 键 词：桔园螨类 WOLBACHIA 基因检测 序列分析 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]