阿米替林对大鼠皮层神经元钠离子通道电流及其mRNA的影响  

THE EFFECTS OF AMYTRIPTYLINE ON ION CHANNEL CURRENTS AND THE mRNA EXPRESSION OF SODIUM CHANNELS IN RAT CORTICAL NEURONS

作　　者：燕兰云[1] 王强[2] 徐武[1] 叶青[1] 肖杭[2] 万琪[1] 

机构地区：[1]南京医科大学第一附属医院神经内科,南京210029 [2]南京医科大学现代毒理学教育部重点实验室,南京210029

出　　处：《中国疼痛医学杂志》2011年第2期111-115,共5页Chinese Journal of Pain Medicine

摘　　要：目的:观察阿米替林(AMI)对大鼠皮层神经元钠通道电流及其mRNA表达的影响。方法:运用全细胞膜片钳模式记录AMI加药前后的钠通道电流(INa);采用实时逆转录聚合酶链反应(real time reverse-transcription-polymerase chain reaction,RT-PCR)观察实验组和对照组皮层神经元钠通道各亚型的mRNA表达量。结果:AMI抑制大鼠皮层神经元INa及其mRNA表达(P<0.05)。10μMAMI显著改变钠通道的激活特性和失活特性。10μMAMI在5 min、10 min及24 h对Nav1.1均有抑制作用,而对于Nav1.2的作用只是在5 min时有抑制作用,对Nav1.6的抑制作用存在波动性的变化。结论:AMI对大鼠皮层神经元INa及其各亚型mRNA的表达均有浓度依赖性抑制作用。AMI对INa的抑制主要通过影响钠通道的激活和失活动力学来实现的,对其各亚型mRNA表达的抑制作用具有不同的特点。Objective： To investigate the effect of amifriptyline（AMI） on INa and the expression of mRNA of sodium channels in cultured rat cortical neurons.Method： The effects of AMI on INa were observed by the whole-cell patch clamp recoding and the mRNA expression of different sodium channels were assessed in experimental and control groups by RT-PCR.Results： AMI significantly inhibited INa and the mRNA expression in rat cortical neurons.AMI of 10 μM significantly changed the activation and inactivation of INa.The inhibition on Nav1.1 was observed at 5 min,10 min and 24 h while Nav1.2 appeared a transient inhibition at 5 min.The inhibiton of Nav1.6 fluctuated at the different time after 10 μM AMI treatment.Conclusion： AMI has a significant inhibitory effect on INa and the expression of mRNA in rat cortical neurons in a concentration-dependent manner.The inhibition of INa was shown by affecting the activation and inactivation of the channel.AMT presented differential effects on the mRNA expression of various subtypes of sodium channels.

关 键 词：偏头痛 皮层扩散抑制 阿米替林 膜片钳 实时逆转录聚合酶链反应 

分 类 号：R747.2[医药卫生—神经病学与精神病学]