RNA干涉Survivin基因对SiHa细胞增殖抑制及凋亡的影响  

Effect of Survivin gene on proliferation inhibition and apoptosis of SiHa cells after RNA interference

作　　者：赵丽晶[1] 徐红梅[1] 刘艳波[2] 梁作文[1] 许多[1] 滕菲[3] 赵秀云[3] 赵淑华[3] 赵雪俭[1] 

机构地区：[1]吉林大学白求恩医学院病理生理学教研室,吉林长春130021 [2]北华大学基础医学院病理生理学教研室 [3]吉林大学第二医院妇产科

出　　处：《中国妇幼保健》2011年第6期900-903,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金〔30670825〕

摘　　要：目的:研究RNA干涉survivin基因对宫颈癌SiHa细胞的增殖抑制及凋亡的影响并探讨其作用机制。方法:重组质粒(pU6-siRNA-Survivin)及空质粒转染SiHa细胞,MTT法检测细胞增殖情况,检测survivin基因转录水平,吖啶噔染色、流式细胞术检测细胞凋亡,免疫组化染色检测survivin、caspase3基因表达。结果:RNA干涉后,抑制SiHa细胞增殖并出现细胞凋亡,survivin基因转录表达下调,caspase3基因表达上调。结论:RNA干涉survivin基因可导致SiHa细胞增殖抑制并凋亡,其机制与caspase3基因表达上调有关。Objective：To study the effect of Survivin gene on proliferation inhibition and apoptosis of SiHa cells after RNA interference and the mechanism.Methods：Recombinant plasmid（pU6-siRNA-Survivin） and empty plasmid were used to transfect SiHa cells,MTT method was used to detect cell proliferation and transcription level of Survivin gene,acridine orange staining and flow cytometry were used to detect cell apoptosis,immunohistochemical staining was used to detect the expressions of Survivin and Caspase 3.Results：After RNA interference,the proliferation of SiHa cells was inhibited,and apoptosis appeared,the transcription of Survivin gene was down-regulated,while the transcription of Caspase 3 was up-regulated.Conclusion：Survivin gene may induce the proliferation inhibition and apoptosis of SiHa cells after RNA interference,the mechanism is related to up-regulation of Caspase 3

关 键 词：宫颈癌 SURVIVIN RNA 干涉凋亡 

分 类 号：R737.33[医药卫生—肿瘤]