不同感觉神经损伤对大鼠病理性疼痛的影响及其机制研究  

Effects and mechanism of different sensory nerve fibers transection on neuropathic pain

作　　者：杨涛[1] 李玉娟[1] 王寿平[1] 车月娟[1] 彭书峻[1] 

机构地区：[1]中山大学孙逸仙纪念医院麻醉科,广州市510120

出　　处：《中华行为医学与脑科学杂志》2011年第2期105-107,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金（30700787）;广东省科技计划社会发展项目（20098030801143）

摘　　要：目的观察剪断大鼠的不同感觉神经分支引起的疼痛行为学表现及脑源性神经营养因子（BDNF）在其中的作用。方法按随机数字表法将24只大鼠分为3组：SUR组，GS组和SHAM组，每组各8只，分别进行剪断Sural、GS神经和假手术。观察3组LSDRG和脊髓背角的BDNF表达情况及损伤的细胞类型。结果GS组大鼠术后同侧后肢机械刺激50％缩足阈值较基础值和SHAM组显著下降（P〈0．01），热刺激撤足持续时间显著延长（P〈0．01），SUR组则无变化（P〉0．05）。GS组LSDRG中BDNF阳性神经元比率[（37．87±4．23）％]和脊髓的BDNF阳性面积百分比[（21．9±3．1）％]较SHAM组增高[（分别为（17．31±2．12）％]，（12．6±1．3）％]，差异具有显著性（P〈0．01）；而SUR组与SHAM组之间无统计学差异（P〉0．05）。GS组表达BDNF的FG阳性神经元较SUR组明显增高（P〈0．01）[分别为（81．5％±3．8）％，（6．4±0．9）％]；GS组中NF200与FG共染神经元占NF200阳性神经元的百分比较SUR组显著增高（P〈0．01）[分别为（47．7±1．8）％和（26．7±2．3）％]。结论切断骨骼肌的感觉神经会产生痛觉过敏，而切断皮肤的感觉神经则不会。不同类型神经元受损，以及I）RG和脊髓背角的BDNF表达差异是导致这种痛觉差异的重要原因。Objective To observe the pain behavioral performance of rats that different sensory nerve fi- bers were transeeted,and examine the expression of brain-derived neurotrophie factor （BDNF） in these models. Methods Twenty-four rats were divided into three groups according to random number table method：SUR group, GS group and SHAM group, which received sural nerve transection, gastrocnemius-soleus nerve transection or sham operation respectively. There were 8 rats in every group. The expression of BDNF in the lumbar 5 DRG and spinal dorsal horn were detected,and the types of damaged cells were also observed. Results In GS group, 50% paw-wlthdrawal thresholds were significantly decreased on the ipsilateral hind paw compared with baseline and with those in SHAM group, and the paw-withdrawal durations in response to the thermal stimulus increased significantly （P〈0.01）. In contrast, no change was found in SUR group（P〉0.05）. The expression of BDNF in the lumbar 5 DRG （ （ 37, 87 ± 4.23 ） % ） and spinal dorsal horn （ （ 2 1. 9 ± 3.1 ） % ） was significantly higher in GS group than in SHAM group（ （ 17.31 ± 2. 12 ） % , （ 12.6 ± 1. 3 ） % ） , and no significant difference was found between SUR and SHAM groups （P 〉 0.05 ）. FG opposite cells which also expressed BDNF in GS group were more than those in SUR group （ （47.7 ± 1.8 ） % and （ 26.7±2.3 ） % ） （P 〈 0.01 ）. The percentage in N200 and FG double positive cells to N200 positive cells in GS group was significantly increased in GS group than those in SUR group （ （47. 7 ± 1.8 ） % , （26.7 ± 2.3 ） % ） （P 〈 0.01 ）. Conclusion The data suggest that injury of the sensory nerve innervating skin does not produce hyperalgesia, but injury of the sensory nerve innerwating muscle does. Different kinds of neuron were damaged and the differences of BDNF expression is essential for this difference.

关 键 词：脑源性神经营养因子 感觉神经 病理性疼痛 

分 类 号：R741[医药卫生—神经病学与精神病学]