脂多糖致肺血管内巨噬细胞分泌功能的改变  

The changes in secretory function of pulmonary intravascular macrophages after challenge of lipopolysaccharide

作　　者：李胜亮 武志宏 张淑琴 秦翠萍 栗涛[2] 陈正堂[2] 金敬顺[2] 马素凤 李佳 

机构地区：[1]解放军第五一七医院呼吸内科,山西岢岚036301 [2]第三军医大学新桥医院全军呼吸内科研究所

出　　处：《中国危重病急救医学》2011年第3期176-178,共3页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目（39800064）

摘　　要：目的 探讨肺血管内巨噬细胞（PIM）在感染性急性肺损伤（ALI）发病中的作用.方法 采用改良Morton法分离、培养猪PIM;用贴壁法获得黏附的PIM,培养于RPMI 1640培养基,予10 mg/L脂多糖（LPS）刺激.用鼠胸腺细胞增殖法测定白细胞介素-1β（IL-1β）活性,酶联免疫吸附法（ELISA）测定IL-6、IL-8含量.结果 LPS刺激后,PIM释放IL-1β、IL-6和IL-8均增多,峰值分别出现在刺激后的2 h[IL-1β活性（10 400±2 389）次/min]、4 h[IL-6含量（0.80±0.36）μg/L]和6 h[IL-8含量（4.94±1.19）μg/L],与刺激前[IL-1β活性（213±85）次/min,IL-6含量（0.27±0.12）μg/L,IL-8含量（1.84±0.53）μg/L]比较差异均有统计学意义（均P＜0.01）.结论 LPS刺激后,PIM分泌多种细胞因子,其中IL-1β升高最早,提示其在ALI发病早期起重要作用;而IL-6、IL-8升高较晚,且持续时间较长,可能对ALI的病情进展起重要作用.细胞因子间的相互作用在ALI的发病中似乎更为重要.Objective To investigate the role of pulmonary intravascular macrophages（PIMs）in the pathogenesis of acute lung injury（ALI）due to infection. Methods Porcine pulmonary blood vessels were flushed by modified Morton method, and PIMs were isolated and cultured. The adhered PIMs were collected with adhesion method and incubated in RPMI 1640 medium. They were challenged with lipopolysaccharide （LPS, 10 mg/L）. The activity of interleukin-1β（IL-1β）, and contents of IL-6 and IL-8 in the culture supernatant were measured by method of thymocyte proliferation and enzyme linked immunoadsorbent assay （ELISA）. Results The released contents of IL-1β, IL-6 and IL-8 from PIMs were increased significantly compared with those before LPS challenge, and they peaked at 2 hours[IL-1β activity：（10 400±2 389）scintillant count/min], 4 hours[IL-6 content：（0. 80± 0. 36）μg/L], and 6 hours[IL-8 content：（4. 94± 1.19）μg/L]after LPS challenge, and the differences were significant compared with those before LPS challenge[IL-1β activity：（213±85）scintillant count/min, IL-6 content：（0. 27 ± 0. 12）μg/L, IL-8 content：（1.84±0.53）μg/L, all P〈0. 01]. Conclusion Among the cytokines released from PIMs after LPS challenge, the increase in IL-1β occurred earlier in comparison with that of IL-6 and IL-8, suggesting that the former might play an important role at the early stage of ALI; on the other hand, though the increase in IL-6 and IL-8 contents occurred later than that of IL-1β but it lasted for a longer duration,suggesting that they might be associated with the advancement of ALI. The results also suggested that interaction of these cytokines played a more important role in the pathogenesis of ALI.

关 键 词：肺血管内巨噬细胞 细胞因子 肺损伤 急性 脂多糖 

分 类 号：R363[医药卫生—病理学]