星点设计-响应面法优化超声提取青竹标多酚  

Study on the Extraction of Polyphenol from Scindapsus officinalis with Ultrasonic Wave Technology Optimized by Central Composite Design-response Surface Method

作　　者：李东东[1] 放茂良[1] 刘琼[1] 李乔丽[1] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院/国家民族事务委员会-教育部共建民族药资源化学重点实验室,云南昆明650500

出　　处：《中药材》2011年第1期129-133,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:利用响应面法优化超声提取青竹标中多酚的工艺条件。方法:在对乙醇浓度、超声时间、液固比单因素考察的基础上,根据星点设计原理进行3因素3水平响应面分析。以FC法测定多酚提取率为响应值,运用响应面法优化提取条件。结果:乙醇浓度为61.14%、超声时间为59.73 min、液固比为27.72(mL/g)(n=3)时,提取率为1.352%,与理论值1.361%的相对误差为-0.66%。结论:超声波提取是种节时、节能、节料的方法,能较好的应用于多酚的提取。星点设计-响应面法优化可得到较好的预测实验结果。Objective：To optimize ultrasonic extraction technology process conditions of polyphenol from Scindapsus officinalis by the response surface method. Methods ： Based on ethanol concentration, ultrasonic time, the liquid-solid ratio of single factor experiment, the principle of design for 3 star factor 3 level response surface methodology was applied. With FC extraction method for determination of polyphenols,the response surface optimization extraction conditions were studied. Results：The ethanol concentration of 61.14 % ,ultrasonic wave extracting time of 59.73 min and the ratio of solvent volume of 27.72：1 （ Extract 3 times） were selected as the optimum conditions,the extraction yield of polyphenols was 1. 352%, with the theoretical 1. 361% for the relative error of -0. 66 %. Conclusion：Ultrasonic extraction is a good method for saving time, energy and material, and can be applied to the polyphenols extraction. Central composite design-response surface optimization can get better ecasting results.

关 键 词：星点设计 响应面法 超声提取 青竹标 多酚 

分 类 号：R282.6[医药卫生—中药学]