粉被玛利亚霉中天然色素的高速逆流色谱法分离制备及质谱分析  

Natural Pigments of Mariannaea puinosa Preparation with High-Speed Counter-Current Chromatography and Analysis with ESI-MS

作　　者：王小董[1] 张德龙[1] 耿广青[1] 李康乐[1] 胡丰林[1] 

机构地区：[1]安徽省微生物防治重点实验室,安徽农业大学,安徽合肥230036

出　　处：《食品与发酵工业》2011年第1期175-178,共4页Food and Fermentation Industries

基　　金：国家自然科学基金项目(30871676);国家863项目(2007AA021506)

摘　　要：采用高速逆流色谱法分离制备粉被玛利亚霉菌丝体甲醇提取物中的天然色素。溶剂体系为V(乙酸乙酯):V(正丁醇):V(甲醇):V(水)=10:3:3:10,以其下相为流动相,上相为固定相,转速850 r/min,流速为2mL/min,紫外检测波长340 nm。所得色素产物的纯度经高效液相色谱分析为95.3%(峰面积归一化法),其分子式由高分辨ESI-MS确认为C26H32O9。经天然产物数据库查询,没有发现与该分子式匹配的化合物,因此该色素可能为一新化合物。High-speed counter-current chromatography was used for separation and purification of natural pigment from the myeelia of Mariannaea puinosa. The tow-phase solvent system was ethyl acetate-n-butanol-methanol-water （10： 3： 3： 10）. The upper phase was stationary phase and the lower phase was mobile phase. The flow-rate of the mobile phase was 2.0 mL/min and the rotation speed was 850 r/min. The effluent of the column was monitored with a UV detector at 340 nm. The purity of the pigment fraction was evaluated by high-performance liquid chromatography （HPLC）. High resolution MS analysis revealed that the molecular formula of the compound was C26H32O9, which was possibly a new compound according to the searching results of Chapman ＆ Hall natural products database.

关 键 词：天然色素 高速逆流色谱 ESI-MS 粉被玛利亚霉 

分 类 号：TS202.3[轻工技术与工程—食品科学]