检测温度对阻抗法快速检测微生物的影响  

The Effect of Temperature on the Rapid Detection of Microorganisms by Impedance

作　　者：张爱萍[1] 吴丹[1] 唐佳妮[1] 侯式娟[2] 刘东红[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029 [2]烟台大学化学生物理工学院,山东烟台264005

出　　处：《中国食品学报》2011年第1期208-213,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：十一五国家科技支撑课题(2009BADB9B06)

摘　　要：检测温度对阻抗参数值及微生物生长代谢影响重大,基于此,本文研究检测温度对阻抗检测的影响。比较无菌利乐培养基及5g/L氯化钠溶液在20、22、24、26、28、30、32、34、36、38、40℃温度下对应的G、θ、Cs、Cp、Z值的变化情况。结果表明这些阻抗参数随温度的改变而呈线性变化,同样的温度波动值,导电性好的培养介质的阻抗参数变化值大;将装有8.4×104CFU/mL藤黄微球菌、3.3×103CFU/mL微生物菌群的测试管分别在20、30、40℃培养,结果在20℃条件下阻抗变化率未能达到检出阈,而当检测温度从30℃升高至40℃,藤黄微球菌的检出时间从11.8h缩短至6.8h,微生物菌群则从16.5h缩短至12.5h,说明检测温度对于阻抗微生物快速检测方法意义重大。The detection temperature has significant impact on impedance parameters value and the growth of micro-bial,based on this,in this paper the effect of temperature on impedance detection was studied.G,θ,Cs,Cp and Z values of aseptic Tetra medium and 5 g/L NaCl solution at 20 ℃,22 ℃,24 ℃,26 ℃,28 ℃,30 ℃,32 ℃,34 ℃,36 ℃,38 ℃,40 ℃ were compared,get linear relationship between these impedance parameters and temperature and the same temperature fluctuations have greater impact on the culture medium of high conductivity.At the same time,we compare the detection time of Micrococcus luteus（8.4×104 CFU/mL）,microbial flora（3.3×103 CFU/mL） grow at 20 ℃,30 ℃,40 ℃ respectively were also compared,and get that when grow at 20 ℃,the change rate of impedance failed to reach the detection threshold,meanwhile when the incubation temperature rise from 30 ℃ to 40 ℃,the detection time of Micrococcus luteus reduced from 11.8 h to 6.8 h,the microbial flora from 16.5 h to 12.5 h,showed that the temperature have significant impact on the impedance rapid method.

关 键 词：阻抗微生物 检测温度 检出时间 

分 类 号：Q93[生物学—微生物学]