Caco-2细胞对酸枣仁皂苷A的跨膜转运  

Transport of Jujuboside A across Caco-2 cell model

作　　者：章新晶[1] 熊淑华[1] 邹霞[1] 

机构地区：[1]南昌大学第二附属医院药剂科,南昌330006

出　　处：《江西医药》2011年第1期8-10,共3页Jiangxi Medical Journal

基　　金：江西省卫生厅中医处课题;编号2007A119

摘　　要：目的研究酸枣仁皂苷A(Jujuboside A,JuA)在Caco-2细胞的跨膜转运特性。方法采用体外培养的Caco-2细胞单层模型,考察时间、介质pH值、药物浓度、抑制剂对JuA在Caco-2细胞上转运的影响。结果 Caco-2细胞转运JuA呈时间及浓度依赖性;在pH为5.0～8.0范围内,Caco-2细胞对JuA的转运不受pH值的影响;P-糖蛋白(P-glycoprotein,P-gp)抑制剂维拉帕米(Verapamil,Ver)对Caco-2细胞转运JuA无影响;线粒体呼吸链复合体Ⅳ抑制剂叠氮化钠(Sodium azide)对Caco-2细胞转运JuA有抑制作用;JuA的AP-BL侧的Papp与BL-AP侧的Papp的两组均数比较无统计学意义。结论 JuA不是P-gp的底物,其跨膜转运是被动转运与主动转运共同参与的过程。Objective To study the transport characteristics of jujuboside A across Caco-2 cell model.Methods Caco-2 cell model was used in vitro to investigate the influence of time,pH,drug concentration,inhibitors on the transport of jujuboside A.Results JuA transporting across Caco-2 cell monolayers has dependence on time and drug concentration,there had no influence on the transport of jujuboside A from 5.0 to 8.0 pH,the inhibitor of P-gp,had no influence on JuA across Caco-2 cell,but Sodium azide the inhibitor of metabolism,can restrain JuA across Caco-2 cell.There had no statistical significance between the Papp from AP to BL and the Papp from BL to AP.Conclusion JuA may not be the substrate of P-gp and passive diffuse and initiative transport may join in the course of transport mechanism of JuA together.

关 键 词：酸枣仁皂苷A CACO-2细胞 P-糖蛋白 跨膜转运 高效液相 

分 类 号：R-331[医药卫生]