RP-HPLC法同时测定宣木瓜中2种三萜酸及其比例  

RP-HPLC Method for Determination of Oleanolic Acid and Ursolic Acid in Chaenomeles speciosa

作　　者：屈晓清[1] 伊芳 聂云[1] 李晋丽[1] 张跃文[1] 丁之恩[1] 

机构地区：[1]安徽农业大学,合肥230036 [2]阜阳市产品质量监督检验所

出　　处：《安徽林业科技》2011年第1期29-34,共6页Anhui Forestry Science and Technology

基　　金：安徽省"十一五"攻关项目(编号:08010302151)

摘　　要：本研究建立了"同时测定宣木瓜中2种三萜酸的含量及其比例"的反相高效液相色谱法。Phenomenex C18色谱柱(250mm×4.6mmi.d.,5μm);柱条件为:柱温25℃;进样量5μL,检测波长210nm。对流动相和流速及精密度展开探讨,同时,采用去杂纯化对测定精密度进行比较研究。研究结果表明:流动相为V乙腈:V纯净水=95:5,流速为0.5mL/min时,齐墩果酸和熊果酸进样量在0.4～2μg范围内,线性关系良好,回归方程分别为:Y=7414804.000X+176597.400,(R2=0.9994);Y=8315634X-2681.667,(R2=0.9998)。得到的2种三萜酸的纯度为93.56%,齐墩果酸和熊果酸的平均回收率分别为98.6%和96.9%,RSD值分别为0.42%和1.2%;2种三萜酸的比例为(2.14～2.22):1,RSD值为1.39%。本方法操作简便,精密度高,重复性好,适用于宣木瓜及其相关原料中齐墩果酸和熊果酸同时定量分析。A new RP-HPLC method for determination of oleanolic acid and ursolic acid in Chaenomeles speciosa was developed.A Phenomenex C18 （250 min×4.6 mm i.d., 5 μm） column was applied; acetonitrile-water （95：5 v/v） was used as the mobile phase at a flow rate of 0.5 mL·min^-1 and monitoring wavelength was at 210 nm. Linear correlation （R2〉0.99） of the peak area and the concentration of the two acids ranged from 0.4 to 2μg was obtained. The relative standard deviation （RSD） of oleanolic acid and ursolic acid were found to be 0.42% and 1.2% respectively. Analysis of laboratory-made mixtures containing known concentrations of oleanolic acid and ursolic acid of Chaenomeles speciosa, as quality control samples, gave a mean recovery percentage of 98.6% and 96.9% respectively. This method is simple, accurate and reproducible. It has wide linear range and can be used for the simultaneous determination of oleanolic acid and ursolic acid in Chaenomeles speciosa.

关 键 词：宣木瓜 齐墩果酸 熊果酸 反相高效液相色谱法 

分 类 号：TQ914.1[化学工程]