野生克氏原螯虾的肌肉生化成分、组织消化酶和免疫酶活性分析  

On muscle biochemical composition,tissue digestive enzymes and immunity-related enzymes of wild Procambarus clarkii

作　　者：于宁[1] 华雪铭[1] 赵朝阳[2] 冯文和 周鑫[2] 王军[1] 朱站英[1] 周洪琪[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种与养殖生物学重点开放实验室,无锡214081 [3]安徽省巢湖市居巢区水产局,巢湖238000

出　　处：《海洋渔业》2011年第1期46-52,共7页Marine Fisheries

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2009JBFB18);上海市教育委员会科研创新项目(11YZ153);上海高校创新团队项目(上海高校创新团队水产动物营养饲料与养殖环境(第二期));上海市重点学科建设项目(Y1101)

摘　　要：实验测定了两种规格(27.0±2.0、12.0±2.0 g)野生克氏原螯虾的肌肉生化成分、组织消化酶和免疫酶活性等。结果表明:大规格虾的肌肉能量以及整虾除肌肉外其它部分的能量均高于小规格虾,肌肉中天冬氨酸、谷氨酸和精氨酸的含量也均高于小规格虾;肝胰脏和肠道内蛋白酶、淀粉酶活力随pH值的增大均呈现先上升后下降的变化规律,两种规格虾肠道中蛋白酶和淀粉酶以及小规格虾肝胰脏中淀粉酶的最适pH值均为7.2,而小规格虾肝胰脏中蛋白酶最适pH值则为7.6;大规格虾血清中血蓝蛋白的含量高于小规格虾,而血清和肝胰脏中超氧化物歧化酶和溶菌酶的活性则都低于小规格虾。Biochemical composition of muscle,digestive enzymes of intestine and hepatopancreas,immunoenzymes of serum and hepatopancreas in two different sizes of wild Procambarus clarkii（27.0±2.0,12.0±2.0 g）.Results showed that the energy of the muscle and the other parts of the large-scale was higher than that of the smaller ones and so were the content of Asp,Glu and Arg in the muscle of the large-scale.The activity of protease and amylase in the hepatopancreas of both sizes rose first and then dropped with the increase of pH.The optimal pH was 7.2 for protease and amylase in intestinal tracts of both sizes,and 7.6 for protease in the hepatopancreas of the small-scale.The content of the hemocyanin of the serum in the large-scale was higher than that of serum in the smalll-scale,while the activity of SOD and lysozyme in the serum and hepatopancreas were lower than that of the smalll-scale.

关 键 词：野生克氏原螯虾 生化成分 蛋白酶 淀粉酶 超氧化物歧化酶 溶菌酶 血蓝蛋白 

分 类 号：Q55[生物学—生物化学]