肝动脉化疗栓塞术对兔VX2肝移植瘤HIF-1α和VEGF表达及肿瘤血管生成的影响  

Effects of TACE on expressions of HIF-1α and VEGF and their correlation with neovascularization in rabbit VX2 liver neoplasms

作　　者：郑延波[1] 刘胜[1] 王云强[1] 李丽君[1] 徐克[2] 

机构地区：[1]烟台毓璜顶医院介入治疗科,山东烟台264000 [2]中国医科大学附属第一医院放射科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2010年第24期2015-2018,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省医药卫生科技发展计划项目(2007HW015);山东省烟台市科学技术发展计划项目(2007139-21)

摘　　要：目的:探讨肝动脉化疗栓塞术对兔VX2肝移植瘤模型肝癌缺氧诱导因子-1α(HIF-1α)和血管内皮细胞生长因子(VEGF)表达的影响及与肿瘤血管生成的相关性。方法:24只VX2荷瘤兔随机分为3组,每组8只。对照组:经肝动脉注入生理盐水2 mL;TAE组:单纯碘油栓塞,UFLP 0.5～0.8 mL;TACE组:碘油抗癌药混悬液栓塞,UFLP0.5～0.8 mL,THP 2 mg。于术后2周应用免疫组织化学法分别检测肿瘤组织中HIF-1α和VEGF的表达,用CD34单克隆抗体标记肿瘤血管内皮细胞,计数肿瘤组织中的MVD。结果:TAE组和TACE组HIF-1α及VEGF表达与MVD值均明显高于对照组,差异有统计学意义(P<0.05),HIF-1α和VEGF的表达与MVD值呈显著正相关(rs=0.537,P<0.01;rs=0.423,P<0.05)。结论:TACE能明显上调HIF-1α的表达,HIF-1α通过密切调控其下级基因VEGF的表达而促进肿瘤血管的生成,影响肝癌的预后。OBJECTIVE：To investigate the effects of transcatheter arterial chemoembolization（TACE） on the expressions of hypoxia-inducible factor-1alpha（HIF-1α） and vascular endothelial growth factor（VEGF） and their correlation with neovascularization in rabbit VX2 liver neoplasms.METHODS：Twenty-four rabbits implanted liver VX2 tumors were randomly divided into 3 groups（n=8）.Control group was administrated with 2 mL NS;the TAE group was administrated with 0.5 to 0.8 mL ultra-fluid lipoidol;the TACE group was administrated with 0.5 to 0.8 mL ultra-fluid lipoidol mixed with 2 mg pirarubicin.Two weeks after treatment,HIF-1α and VEGF expressions were detected by immunohistochemistry,and the microvessel density（MVD）of the section marked with CD34 antibody was calculated.RESULTS：The expressions of HIF-1α and VEGF and MVD in the TAE group and TACE group were stronger and higher than those in the control group（P〈0.05）;HIF-1α was positively correlated with VEGF and MVD（rs=0.537,P〈0.01;rs=0.423,P〈0.05）.CONCLUSION：Significant overexpression of HIF-1α is found after TAE and TACE.HIF-1α plays an important role in the prognosis of liver neoplasms by up-regulating the expression of VEGF and increasing MVD.

关 键 词：肝肿瘤 兔 缺氧诱导因子-1α 血管内皮生长因子 新生血管化 免疫组织化学法 

分 类 号：R735.7[医药卫生—肿瘤]