三氯生和三氯卡班对人体肝细胞DNA损伤的研究  

The genotoxicity of triclosan and triclocarban in human hepatocyte LO2 cell

作　　者：李林朋[1,2] 马慧敏[1] 胡俊杰[1,2] 黎玉华[1] 王艺陪[3] 盛国英[1,3] 傅家谟[1,3] 

机构地区：[1]中国科学院广州地球化学研究所//有机地球化学国家重点实验室//广东省环境资源利用与保护重点实验室,广东广州510640 [2]中国科学院研究生院,北京100049 [3]上海大学环境与化学工程学院//环境污染与健康研究所,上海200444

出　　处：《生态环境学报》2010年第12期2897-2901,共5页Ecology and Environmental Sciences

基　　金：国家自然科学基金(NSFC-21007072);中国科学院广州地球化学研究所知识创新领域前沿项目(GIGCX-09-01);中国科学院知识创新工程重要方向项目(KZCX2-YW-GJ02);中国科学院知识创新工程基金(KZCX2-YW-403)

摘　　要：三氯生(TCS)和三氯卡班(TCC)作为优良的抗菌和消毒剂,在药物和个人护理用品中广泛使用。近几年来,TCS和TCC在各种环境介质和生物体中被频繁检出,并引起国内外环境科学工作者的重点关注。目前,有关TCS和TCC对水生生物的毒性研究已有大量的报道,其对人体健康危害的报道仍较少。为了评估TCS和TCC对人体的遗传毒性,利用彗星实验研究两种污染物对正常人肝细胞(L02)DNA的损伤效应。结果发现:三氯生和三氯卡班皆能引起人体肝细胞的DNA损伤,并呈现显著的剂量-效应关系和时间效应关系。相比于TCS而言,L02细胞对TCC更为敏感,在低暴露剂量(2.38μmol.L-1)下就可以引起DNA断裂损伤。该结果有助于进一步揭示TCS和TCC对人体毒害的机制,更全面的评估两种污染物对人体健康的风险。Triclosan（TCS） and triclocarban（TCC） were excellent antibacterial agents,and they have been widely used in pharmaceutical and personal care products.Because TCS and TCC have been frequently detected in different types of environmental matrix and in biological samples,more and more attentions were payed about their potential detrimental effects to human health.To date,several reports were focused on the toxicity of TCS and TCC to the aquatic organisms,the research about their adverse effect to our human being is rare.Base on the advantage of single cell gel electrophoresis（comet assay）,the DNA damage potential of TCS and TCC in human hepatocyte（L02） cell have been evaluated.The results showed that both TCC and TCS markedly increased DNA damage in time-and dose-dependent manners.And compared with TCS,L02 cell is more sensitive to TCC,only exposed to 2.38 μmol·L-1 of TCC,statistically significant DNA damage were observed in L02 cells.These results could be helpful to calrify the mechanism of TCS and TCC induced toxicity and evaluate the human health risk comprehensively.

关 键 词：三氯生 三氯卡班 彗星实验 正常人肝细胞(L02) DNA损伤 

分 类 号：X171.5[环境科学与工程—环境科学]