两种方法定位5个地点大豆蛋白质含量QTL  

QTL analysis of soybean protein content using two methods in 5 different environments

作　　者：单大鹏[1,2,3] 刘春燕[1] 蒋洪蔚[1] 董晓慧 陈庆山[1,2] 胡国华[1,2] 

机构地区：[1]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [2]东北农业大学,黑龙江哈尔滨150030 [3]黑龙江省农科院绥化分院,黑龙江绥化152052

出　　处：《中国油料作物学报》2011年第1期9-14,共6页Chinese Journal of Oil Crop Sciences

基　　金：转基因专项大豆导入系构建及有利隐蔽基因挖掘(2009ZX08009-013B);农业部"引进国际先进农业科学技术"(2006G01);国家863计划(2006AA10Z1F4)

摘　　要：利用CIM和MIM法,对大豆Charleston×东农594的154个重组自交系在5个不同地点进行蛋白质含量测定和QTL定位分析。共检测到25个QTL,分别位于A1、B1、C2、D1a、D1b、E、J、L和N连锁群上。用CIM法定位了20个与蛋白质含量相关的QTL,用MIM法定位了9个与蛋白质含量相关的QTL。在C2连锁群上多次定位的ProC2-4,标记区间为Sat_092-Satt460,与前人找到的QTL位点一致。Two mapping methods,composite interval mapping（CIM） and multiple interval mapping（MIM）,were employed to detect quantitative trait loci（QTL） of soybean protein content using 154 recombination inbred lines（RIL） population derived from a cross between Charleston and Dongnong 594 in five different locations.As a result,25 QTLs for protein content were mapped in linkage group A1,B1,C2,D1a,D1b,E,J,L,and N.In total,20 QTLs for protein content were detected by CIM,and 9 QTLs detected by MIM.Among the QTLs,ProC2-4 was mapped multiple times between Sat_092 and Satt460 on C2 linkage group,referred as a main effect QTL for soybean protein content.

关 键 词：大豆 蛋白质含量 QTL 多个环境 复合区间作图法 多重区间作图法 

分 类 号：S565.103[农业科学—作物学]