培养条件对紫苏下胚轴外植体再生的影响  

Effect of culture conditions on shoot regeneration of hypocotyl explants from Perilla frutescens

作　　者：刘培培[1] 李会珍[1] 张志军[1] 刘西亮[1] 

机构地区：[1]中北大学化工与环境学院生命科学系,山西太原030051

出　　处：《中国油料作物学报》2011年第1期83-86,共4页Chinese Journal of Oil Crop Sciences

基　　金：山西省回国留学人员科研资助项目(2009065)

摘　　要：以紫苏下胚轴为材料,研究紫苏外植体芽再生的影响因素。结果表明:苗龄、黑暗时间、激素浓度及配比对紫苏芽再生均有较大影响。取13d苗龄的下胚轴先在分化培养基(MS+3.0mg/L BA+0.3mg/L NAA)黑暗预培养8d后转为14h/d光照条件培养可获得较高的再生频率。诱导芽苗生根最佳培养基为1/2MS+1.0mg/L BA+0.3mg/L IAA。Culture conditions affecting regeneration of hypocotyls from Perilla frutescens were investigated.The results showed that seedling age,preculture time in darkness and hormone concentrations had important influences on regeneration ratio of shoots.The highest ratio of regeneration was realized when the hypocotyls of 13-days seedling were precultured for 8 days in darkness and then cultured under the condition of 14h/d light in the medium of MS＋3.0mg/L BA＋0.3mg/L NAA.The optimal medium for inducing root from regeneration shoot was 1/2MS＋1.0mg/L BA＋0.3mg/L IAA.

关 键 词：紫苏 下胚轴 再生 

分 类 号：S565.8[农业科学—作物学]