猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断  

Concentration Changes of Potassium and Hypoxanthine in Vitreous Humor of Swine and Its Application to Postmortem Interval Estimation

作　　者：方超[1] 王松才[2] 孙立敏[2] 张小婷[2] 龙伟清[3] 竞花兰[1] 

机构地区：[1]中山大学中山医学院法医病理教研室,广东广州510080 [2]广州市刑事科学技术研究所,广东广州510030 [3]中山大学附属第一医院检验科,广东广州510080

出　　处：《法医学杂志》2011年第1期9-12,21,共5页Journal of Forensic Medicine

摘　　要：目的研究猪眼玻璃体液成分变化及其与死亡时间(postmortem interval,PMI)的关系。方法放血处死家猪后取带眼睑的眼球96只,随机分为24组,在避光、温度(15±2)℃、湿度(50±5)%条件下分别放置2～96h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤(hypoxan-thine,Hx)浓度;用SPSS统计软件检测数据进行统计分析。结果玻璃体液中K+、Hx的浓度与PMI呈线性相关,R2分别为0.767和0.793;K+和Hx两者浓度与PMI经二元线性回归分析,R2为0.866;玻璃体液中Na+、Cl-浓度与PMI无明显相关性。结论玻璃体液K+、Hx浓度变化可作为较为客观的推断PMI的参考指标,两者与PMI的二元回归方程推断死亡时间有更高的准确性。Objective To investigate the relationship between postmortem interval（PMI） and concentration changes of components in swine vitreous humor.Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage,being randomly divided into 24 groups,were stored in dark situation,at temperature of（15±2） ℃ and humidity of（50±5）% for 2-96 hours separately.The vitreous humor was collected.Concentrations of K＋,Na＋,Cl-and hypoxanthine（Hx） were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph（UPLC）.The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K＋ and Hx were positively correlated with PMI（R2=0.767 and R2=0.793,respectively）.Binary linear regression showed a higher correlation for K＋ and Hx with PMI estimation（R2=0.866）.PMI was not significantly correlated with vitreous Na＋ and Cl-concentrations.Conclusion Vitreous K＋ and Hx concentrations can be used as the objective markers for PMI estimation.The binary linear regression functions of vitreous K＋ and Hx concentrations with PMI are more accurate for estimating the PMI.

关 键 词：法医病理学 次黄嘌呤 钾 玻璃体液 死亡时间 猪 

分 类 号：R576[医药卫生—消化系统]