氟致小鼠肾脏组织形态结构损伤的观察  

THE OBSERVATION OF STRUCTURAL LESIONS OF MOUSE RENAL TISSUE INDUCED BY FLUORIDE

作　　者：吴培福[1] 陈粉粉[1] 潘绍发[1] 曲伟杰[2] 

机构地区：[1]西南林学院保护生物学学院,云南昆明650224 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《山东农业大学学报（自然科学版）》2011年第1期75-78,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：云南高端人才引进计划(2009CI125);云南省教育厅科学研究基金(09Y0289)

摘　　要：为研究氟致非骨相组织的损伤作用,选用20只20日龄的昆明小鼠,随机分为4组,每组5只,分别饲喂含0 mg/L、50 mg/L1、00 mg/L和150 mg/L氟化钠的饮水120 d,并通过石蜡组织切片观察氟对肾脏的损伤作用。研究结果表明:50 mg/L氟中毒组小鼠肾脏肾小管排列结构无异常变化,肾小球细胞出现空泡样病变。100 mg/L氟中毒组小鼠肾脏肾小管排列结构没有明显的异常,肾小球内皮细胞和肾小管上皮细胞出现空泡样变,并出现微核现象和凋亡样细胞,且近曲小管上皮细胞有脱落现象。150 mg/L氟中毒组小鼠肾脏肾小管排列结构轻微紊乱,肾小球内皮细胞和肾小管上皮细胞发生变性、坏死,肾小管内脱落的上皮细胞增多。In order to analyse toxic effect of fluoride on non - skeletal tissue, 20 Kunming mice with age of 20 days old were selected, and randomly divided into 4 groups with each group 5 mice. The animals were feed respectively with water containing 0 mg/L, 50 mg/L, 100 mg/L and 150 mg/L sodium fluoride. The lesions of renal tissue induced by fluoride were studied by paraffin -embedded tissue specimens. The results showed that the renal tubule has normal arrangement, but vacuolated changes were found within glomerular endothelium in 50 mg/L fluoride -treated group; that the kidney tubule has normal situation as well as vacuolated lesions within glomerular endothelium and tubular epithelium, micronuclei and apoptosis -like cells accompanied by shedding off of tubular epithelium in 100 mg/L fluoride -treated group; that renal tubule has slight dearrangement, degeneration and necrosis of glomerular endothelium and tubular epithelium was observed, and the amount of tubu- lar epithelium shed off from tubule are higher in 150 mg/L fluoride - treated group.

关 键 词：氟 昆明小鼠 肾 组织切片 形态结构 

分 类 号：R-33[医药卫生]