rhTPO对CD_(34)^+细胞增殖及形成CFU-MK作用的研究  

EFFECT OF rhTPO ON THE PROLIFERATION AND FORM CFU-MK OF CD_(34)^+ CELLS

作　　者：宁丰[1] 王良绪[2] 尉达民 陈萍生 

机构地区：[1]北京军区总医院,北京市东城区南门仓5号100700 [2]北京医科大学第三医院,北京市海淀区学院路100083

出　　处：《白血病》1999年第4期207-209,共3页

摘　　要：目的研究重组人TPO（rhTPO）单独及与rhIL-3、rhIL－6、rhSCF协同体外对巨核系的增殖效应。方法采用纯化的CD+34细胞，进行求固体巨核细胞集落培养和液体悬浮培养，研究TPO及与IL－3、IL－6、SCF协同体外对CD+34细胞增殖及向巨核系空向分化的特性，和形成CFU－MK的作用。结果rhTPO可以诱导CD+34细胞形成CFU－MK，增加巨核细胞集落数，这种作用可以被rhIL－3、rhIL－6、rhSCF增强。液体培养中，rhTPO促进CD+34变细胞分化／成熟为表达GPⅡb／Ⅱa的细胞，增加体系中CD+34。细胞，rhIL-3、rhIL－6、rhSCF可以协同TPO的作用。结论TPO作为一种促增殖因子，促进巨核系祖细胞的增殖及分化。Objective To stady the effecf of rhTPO on proliferation and from CFU-MK of CD34 cells Methods It thrombopoietin (TPO) is the ma jor regulator of megakaryocytopoiesis and platelet production in vivo. We examined the effects of recombinante human TPO in combination with IL-3 IL-6, SCD on megakaryocytopoiesis in vitro. Results rhTPO supported the growth of CFU-MK of CD34 cells,increase the numbers of CFU-MK- This kind of effects can be further enhanced by lL-3, lL-6 and SCF. In liquid culture, rhTPO increase the riumbers of CD6. cells, CD+34 cells differentiate/mature to CD+34. cells which express GP E b/ N a. IL-3,IL-6 and SCF can synergize with TPO. Conclusion TPO stimulate proliferation and maturation of megakaryocyte progenitor.

关 键 词：促血小板生成素 CD^+34 巨核细胞集落 

分 类 号：R733.1[医药卫生—肿瘤] R730.51[医药卫生—临床医学]