长春新碱、甲基强的松龙诱导急性淋巴细胞白血病细胞凋亡  

APOPTOSIS IN FRESH ACUTE LYMPHOBLASTIC LEUKEMIA CELLS INDUCED BYVINCRISTINE OR METHYLPREDNISOLONE

作　　者：唐加明 陈安薇 李濠德 黄仁魏[2] 李铭权[2] 

机构地区：[1]广州市第二人民医院,广州市多宝路63号510150 [2]中山医科大学附属第三医院,广州市石牌岗顶510630

出　　处：《白血病》1999年第4期214-216,共3页

摘　　要：目的了解长春新碱（VCR）、甲基强的松龙（mPSL）诱导原代急性淋巴细胞白血病（ALL）细胞凋亡及其规律。方法应用细胞形态学检查，二苯胺法DNA片断化定量及DNA凝胶电泳方法分析检测。结果发现ALL细胞体外孵育24h，空白组DNA片断率为（169±4．6）％，加VCR1μM组和mPSL1μM组DNA片断率分别为（50．7±9．7）％（P＜0．05，n＝12）和（37.5±11．7）％（P＜0．05，n＝12）。两种药物诱导DNA片断化均呈剂量和时间依赖性。光镜下检查见细胞核固缩、碎裂，凋亡小体形成。DNA电泳显示典型的DNAladder，其亮度随剂量增加或时间延长而增强。结论VCR和mPSL诱导ALL细胞出现凋亡，可能是其抗白血病的机制之一。Objective The present study was to investigate apoptosis in fresh acute lymphoblastic leukemia (ALL)cells induced by vincristine (VCR) or methylprednisolone (mPSL) Methods By checking the morphology of apoptotic cells, quantitation of DNA fragmentation by means of the diphenylamine reaction,agarose gel electrophoresis of DNA fragmentation- Results The percentage of DNA fragmentation was (16. 9 ±4.6) % after the cells were incubated without a apoptosis-inducing reagent. After the cells were treated with luM VCR or 1uM mPSL for 24 hours,the percentage of DNA fragmentation was (50. 4±9. 7) % (P＜0.05, n=12) and (37. 5±11. 7 )% (P＜0.05, n=12 ), respectively. Induction of DNA fragmentation by VCR or mPSL was both dose-and time-dependent. Chromatin condensati0n, nuclear fragmentation and production of apoptotic bodies were seen in the cells treated with VCR or mPSL by light microscopy- DNA electrophoresis of VCR or mPSL treated cells showed nuclear fragmentation in oligonucleosomal fragments (DNA ladder) whic was both dose-and time-dependent. Conclusion VCR or mPSL induce apoptosis in ALL cells. It is one of the mechanisms through which VCR or mPSL kill ALL cells.

关 键 词：细胞凋亡 甲基强的松龙 白血病 药物疗法 

分 类 号：R733.710.5[医药卫生—肿瘤]