大麻素受体1在慢性乙型肝炎患者肝组织表达及与血清转化生长因子-β1和瘦素的关系  

Expression of cannabinoid receptor 1 in liver tissue of patients with chronic hepatitis B and its relationship with the serum levels of TGF-β1 and Leptin

作　　者：叶立红[1] 王翀奎[2] 刘玉珍[1] 卢建华[1] 侯军良[1] 高会霞[1] 杨莉[1] 赵召霞[1] 李兵顺[1] 戴二黑[1] 

机构地区：[1]河北省石家庄市第五医院检验科,050021 [2]河北医科大学西山校区教务处

出　　处：《中国综合临床》2011年第2期117-120,共4页Clinical Medicine of China

基　　金：中国肝炎防治基金会王宝恩肝纤维化研究基金项目（20080013）

摘　　要：目的探讨大麻素受体1（CB1）在慢性乙型肝炎（CHB）患者肝组织中定位表达及其与肝纤维化评分、血清转化生长因子（TGF）-β1、瘦素（Leptin）水平的相关性。方法选择118例CHB患者进行肝组织活检，通过免疫组织化学染色检测肝组织中CB1表达并进行CB1半定量评分。采用双抗体夹心ABC-ELISA法检测血清TGF-β1和Leptin含量。据CB1表达量分为4组：（-）组、（＋）组、（＋＋）组、（＋＋＋）组。结果随着肝组织CB1阳性表达量的不断增加，肝纤维化评分逐渐升高，各组间差异有统计学意义（F=23．369，P=0．000）；血清TGF-β1和Leptin水平也逐渐升高，各组间差异有统计学意义（F值分别为8．762和5．749，P值分别为0．001和0．027）。结论CHB肝组织损伤可使肌纤维母细胞表达CB1增强，CB1可能通过与TGF-β1和Leptin共同作用，诱导肝星状细胞的增殖、活化，导致肝纤维化不断进展。Objective To observe expression and location of cannabinoid receptor 1 （CBI） in liver tissue of patients with chronic hepatitis B （ CHB ）, and analyze the relationship of it with the liver fibrosis score, the serum levels of TGF-β1 and Leptin. Methods Liver biopsies were performed in 118 patients with CHB. The expression of CB1 in liver tissue was observed by immune histochemical staining, and semi-quantitative analysis was carried out to devide the CB1 score into four grades - , ＋ , ＋ ＋, ＋ ＋ ＋. Serum levels of TGF-β1 and Leptin were determined by ABC-ELISA double-antibody sandwich method. Results The expression of CB1 in liver tissue with CHB had significant relationship with the fibrosis score. As the expression of the CB1 increased, the fibrosis score became higher （ F = 23. 369,P = 0. 000）. Moreover, the expression of CB1 in liver tissue with CHB had significant relationship with the serum levels of TGF-β1 and Leptin（ F values were 8. 762 and 5.749 ;P values were 0. 001 and 0. 027, respectively）. Conclusion CB1 may play promotive role in the process of hepatic fibrosis through regulation of TGF-β1 and Leptin.

关 键 词：慢性乙型肝炎 大麻素受体1 肝纤维化 转化生长因子Β1 瘦素 

分 类 号：R575.2[医药卫生—消化系统]