不同波长下高效液相色谱检测胭脂红酸的比较研究  

Study on high performance liquid chromatography determining carminic acid at two wavelength

作　　者：郭元亨[1] 马李一[1] 郑华[1] 张弘[1] 甘瑾[1] 张忠和[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所/国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《广东农业科学》2011年第2期102-105,共4页Guangdong Agricultural Sciences

基　　金：科技部成果转化项目(2007GB24320425);国家林业科技成果推广项目([2010]11)

摘　　要：为建立高效液相色谱测定胭脂红酸的方法,通过比较不同配比的流动相,不同流速和温度对出峰效果的影响,发现流动相为甲醇-乙腈-磷酸(0.01%)的混合液(体积比为10∶15∶75),流速为0.8 mL/min,柱温为20℃时,出峰效果最好。纯度为100%的胭脂红酸作为标准样品,绘制标准曲线,280 nm和494 nm的检测波长下分别得回归方程Y=60.7360X,R2=0.9999;Y=8.6048X,R2=0.9990,两者线性关系均良好。以280 nm和494 nm为检测波长,使用纯度为100%的胭脂红酸验证仪器的精密度,自制胭脂虫红色素验证稳定性和加标回收率,测得精密度相对标准偏差分别为0.42%和0.54%,稳定性相对标准偏差分别为0.16%和0.32%,加标回收率分别为96.8%和94.3%。两种检测波长均可用于胭脂红酸的定量分析。This study aimed to establish the method on high performance liquid chromatography determining carminic acid.Comparing the different kinds of mobile phase,different flow rate and temperature of the column.The result showed that when mobile phase was consist of acetonitrile,methanol and phosphoric acid（0.01%）（volume ratio 10∶15∶75）,flow rate was 0.8 mL/min,column temperature at 20℃,peak shape was the best.This mothed used for determined carminic acid both at 280 nm and 494 nm.Carminic acid（purity：100%） was utilized as standard sample,made standard curve,obtained regression equation Y=8.6048X,R2=0.9990,and Y=60.7360X,R2=0.9999,respectively.Both equation＇s linear relationship were fine.When determining cochineal red pigment,the result showed that by this method,sample had good stability（determined at 280 nm,RSD=0.16%;at 494 nm,RSD= 0.32%）,equipment had high precision（determined at 280 nm,RSD=0.42%;at 494 nm,RSD=0.54%） and sample had high recovery rate（determined at 280 nm,recovery rate was 96.8%,at 494 nm was 94.3%）.High performance liquid chromatography was an ideal method for carminic acid determination,especially chose the detect wavelength at 280 nm.

关 键 词：胭脂红酸 高效液相色谱 测定方法 

分 类 号：TS202.3[轻工技术与工程—食品科学] S899.3[轻工技术与工程—食品科学与工程]