白桦细胞悬浮培养产三萜及其营养成分消耗的动态  

Kinetics of Triterpenoid Production and Nutrient Consumption in Birch (Betula platyphylla) Suspension Cell

作　　者：范桂枝[1] 翟俏丽[1] 于海娣[1] 王晓东[1] 王博[1] 詹亚光[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《林业科学》2011年第1期62-67,共6页Scientia Silvae Sinicae

基　　金：中央高校基本科研业务专项资金(DL09BA05);黑龙江省自然科学基金重点项目(ZD200918);中国博士后基金(20080440136)

摘　　要：以产三萜的白桦悬浮系为试验材料,在优化白桦悬浮细胞生长与三萜积累的培养基种类和接种量的基础上,分析白桦悬浮细胞生长和三萜积累的动力学关系,同时考察1个培养周期内白桦细胞内外主要营养成分的变化。结果表明:1)在MS,NT,IS,WPM,1/2MS,B56种培养基和10～40g·L-1接种量中,有利于白桦细胞生长和三萜生产的培养基为B5和接种量为20～30g·L-1FW;2)在1个培养周期内,白桦悬浮细胞培养过程中三萜的产量与细胞生物量是相偶联的,随着生物量的增加三萜产量呈增长趋势,其中,白桦细胞在第12天比生长速率达到最高0.18,三萜合成速率和比合成速率在第9天达到最高值,分别为0.73mg·g-1和0.06;3)在白桦细胞培养的1个周期内,培养液中的蔗糖,NO3-,NH4+和PO43-在细胞培养的第9天基本被消耗掉,而细胞内的营养物质,除NH4+在第3天达最高值外,其他营养成分均在第6天达最大值。由此可推断:培养液中营养成分的不足限制了细胞的快速增值和三萜含量的提高。Effects of the basic media and inoculum size on birch cell growth and the triterpenoid production were investigated using birch suspension cells,on this basis,the kinetic relationships of birch cell growth,triterpenoid production and nutrient consumption were also analyzed.The results showed the opbimal conditions for the birch cell growth and triterpenoid production were 20 ～ 30 g·L ^-1 （FW） of inoculum size,in B5 medium.The kinetics analysis for birch cell suspension culture showed that triterpenoid production was increasing with the birch cell growth during a cell subculture cycle.During the teriod,the maximum specific growth rate was 0.18 on the 12^th day,and the maximum triterpenoid synthesis rate was 0.73 mg· g^ -1 on the 9th day.Sugar,NO3^- ,NH 4^＋ and PO4^3 -in the culture medium were nearly used up on the 9^th day,The content of sugar,NO 3^- ,and PO4^3 -in birch cell were highest on 6^th day,but the content of NH 4^＋ in birch cell were higher on 3^th day.The results snggested that the nutrient deficiencies in medium wonld limited the cell rapid multiplication and triterpenoid content accumulation.

关 键 词：白桦 悬浮细胞 三萜 营养成分 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]