应用MADB-106乳腺癌细胞构建大鼠转移性骨癌痛模型  

A rat model of tibia cancer pain produced by MADB-106 mammary gland carcinoma cells

作　　者：李华艳[1] 杨保仲[2] 聂丽霞[2] 潘喻飞[1] 王春燕[2] 张彦清[2] 

机构地区：[1]山西医科大学麻醉学系,太原030001 [2]山西医科大学第一医院麻醉科,太原030001

出　　处：《肿瘤研究与临床》2011年第2期85-87,共3页Cancer Research and Clinic

基　　金：基金项目：山西省科技攻关计划（20090311058-2）

摘　　要：目的应用MADB-106大鼠乳腺癌细胞构建转移性骨癌痛模型，通过对该模型痛行为学、影像学、组织学观察等检测进行治疗研究。方法将MADB-106大鼠乳腺癌细胞接种到SD大鼠胫骨上段骨髓腔内，构建骨癌痛动物模型。以大鼠自发性缩足反射次数评估大鼠自发性痛情况，以自由活动时下肢跛行程度评分观察大鼠行走诱发痛，以热痛觉阈值观察大鼠热痛觉过敏情况，以X线、骨组织病理学检测观察癌细胞对骨结构的破坏程度。结果造模组大鼠术后第15天自发缩足反射次数为（14．42±1．24）次，第22天为（18．33±1．37）次，第25天为（21．25±1．54）次，与术前比较差异有统计学意义（均P〈0．001）；术后第15天热刺激痛觉阈值为（11．86±1．63）S，第22天为（8．38±1．05）S，第25天为（7．47±1．25）s（P〈0．001）；术后第15天自由行走痛评分为（1．25±0．62）分，第22天为（2．00±0．95）分，第25天为（2．33±1．07）分。术后第15天开始出现明显的自发性痛、热痛觉过敏和行走诱发痛，胫骨X线片显示明显的骨破坏，组织学研究显示骨髓腔内肿瘤生长，向外侵蚀破坏骨皮质。结论成功应用MADB-106乳腺癌细胞构建大鼠骨癌痛模型，造模2周后可以用于癌痛治疗的评价。Objective To establish a tibial cancer pain model with MADB-106 mammary gland carcinoma cell line and to conduct therapeutic research through the behavior pain, X-ray, histological observation of the model. Methods A rat model of bone cancer pain was established by intra-tibial inoculations of MADB-106 rat mammary gland carcinoma cells in SD rats. Spontaneous pain was assessed by the reflection of spontaneous paw withdraw, move-evoked pain was observed by the extent of lower extremity claudication when the rats walked and heat hyperalgesia was evaluated by using a thermal doiorimeter. The structural damage of the bone was monitored by X-ray and histology. Results The model group spontaneously withdrawed their paws （14.42±1.24） times on the 15th day after operation （P 〈0.001）, （18.33±1.37） times on the 22nd day （P 〈0.001）, （21.25±1.54） times on the 25th day （P 〈0.001）. The radiant pain thresholds of the model group was （11.86±1.63） s on the 15th day after operation （P 〈0.001）, （8.38±1.05） s on the 22nd day （P 〈 0.001）, （7.47±1.25） times on the 25th day （P 〈0.001）. The move-evoked pain score of the model group was （1.25±0.62） on the 15th day after operation （P 〈0.001）, （2.00±0.95） on the 22nd day （P 〈0.001）, （2.33±1.07） on the 25th day （P 〈0.001）. The results showed that rats of the model group displayed the gradual development of spontaneous pain, heat hyperalgesia and move-evoked pain on the 15-25 days after injection of the tumor cells. The X-ray of the tibia showed clear bone destruction. The histology of the bone showed the bone marrow cavity was full of tumor cells and the cortical bone had been destroyed. Conclusion The bone cancer model has been built well and it will be useful to evaluate the therapy of cancer pain after two weeks.

关 键 词：乳腺肿瘤 肿瘤转移 骨骼 动物模型 疼痛测定 

分 类 号：R-332[医药卫生]