人参皂苷Rg3对人类乳腺癌MCF-7细胞Cx26基因表达及细胞间隙连接通讯功能的影响  

Effect of ginsenoside Rg3 on Cx26 gene expression and gap junctional intercellular communication inhuman breast cancer cell line MCF-7

作　　者：马艳梅[1] 温文斌[2] 刘基巍[3] 

机构地区：[1]长治医学院附属和济医院消化内科,046000 [2]长治医学院附属和济医院肾内科,046000 [3]大连医科大学附属第一医院肿瘤科,046000

出　　处：《肿瘤研究与临床》2011年第2期91-93,共3页Cancer Research and Clinic

基　　金：基金项目：国家自然科学基金（30672753）

摘　　要：目的研究人参皂苷Rg3对人类乳腺癌细胞MCF-7增殖抑制作用、连接蛋白cx26基因表达及对细胞间隙连接通讯（GJIC）功能的影响。方法使用含不同浓度人参皂苷Rg3培养基体外培养MCF-7细胞24h，并设正常培养条件下MCF-7细胞为对照组，采用四甲基偶氮唑蓝（MTF）法检测人参皂苷Rg3各浓度下MCF-7细胞增殖活力，并选取能明显抑制MCF．7细胞增殖的人参皂苷Rg3浓度组为实验组。RT—PCR检测各实验组和对照组连接蛋白Cx26 mRNA的表达。通过划痕标记荧光传输实验检测上述各组MCF，7细胞GJIC功能的恢复情况。结果在含人参皂苷Rg310、20、40、80、160斗g／ml体外培养MCF-7细胞24h后，MCF-7细胞增殖的抑制率分别为3．1％、5．2％、16．0％、26.3％、29．1％。与对照组比较，质量浓度在40μg／ml以上的人参皂苷Rg3均可明显抑制肿瘤细胞增殖（P〈0．05），故选取含40、80、160斗g／m1人参皂苷Rg3MCF-7细胞组为实验组。在各实验组中，随人参皂苷Rg3质量浓度的增大，Cx26 mRNA表达逐渐增强，与对照组相比，差异有统计学意义（P〈0．05）。荧光黄染料划痕标记荧光传输实验显示，对照组荧光黄染料荧光染色仅限于单个细胞，各实验组荧光黄染料荧光可通过细胞间隙连接传输至相邻细胞，形成片状荧光染色。结论一定浓度人参皂苷Rg3可增强人类乳腺癌细胞MCF-7 Cx26基因表达，恢复其GJIc功能，这可能是人参皂苷Rg3抑制MCF-7细胞增殖，发挥抗肿瘤作用机制之一。Objective To study the function of ginsenoside Rg3 on proliferation in human breast cancer cell line MCF-7 and effect of ginsenoside Rg3 on Cx26 gene expression and gap junctional intercellular communication （GJIC） in MCF-7, cultured in vitro. Methods Human breast cancer cells MCF-7 was exposed to ginsenoside Rg3 at differential concentrations for 24 h, respectively. The cell proliferation inhibition was measured by 3-[4,5-dimethylthiazo-2-yl]-2,5 diphenyl tetrazolium bromide （MrlT） assay. The expression of Cx26 mRNA was measured by RT-PCR in experimental groups and control group. The GJIC function of MCF-7 cell was examined with scrape-loading dye transfer assay. Results Human breast cancer cell line MCF-7 was exposed to ginsenoside Rg3 at a concentration of 10, 20, 40, 80, 160 Ixg/ml, respectively. The inhibition ratio was 3.1%, 5.2 %, 16.0 %, 26.3 %, 29.1% respectively after 24 h. Compared with control group, the concentration of 40 μg/ml above could significantly inhibit MCF-7 cell proliferation （P 〈0.05）, so the experimental groups were exposed to ginsenoside Rg3 at a concentration of 40, 80, 160 μg/ml, respectively. The expression of Cx26 mRNA in every experimental group compared with control group was enhanced when MCF-7 cell was exposed to ginsenoside Rg3 at a higher concentration. It was observed that Lucifer yellow fluorescent staining was limited to a single cell in control group through fluorescent microscope, but Lucifer yellow fluorescent transfered through gap junction cells to neighboring cells, then came into being flake fluorescent staining in experiment groups. Conclusion Ginsenoside Rg3 can enhance the expression of Cx26 mRNA in MCF-7 cell and restore the gap junctional intercellular communication, which may be one of important mechanisms of ginsenoside Rg3 in antitumor.

关 键 词：乳腺肿瘤 细胞系 肿瘤 药物筛选试验 抗肿瘤 连接蛋白类 

分 类 号：R737.9[医药卫生—肿瘤]