双表型急性白血病流式细胞术免疫分型检测结果  

Immunophenotype of double acute leukemia detected by flow cytometry

作　　者：要跟东[1] 霍红旗[2] 李鹏[2] 刘爱民[2] 

机构地区：[1]邯郸市中心医院生物医学室,河北省056001 [2]邯郸市中心医院核医学科,河北省056001

出　　处：《白血病．淋巴瘤》2011年第2期103-104,共2页Journal of Leukemia & Lymphoma

摘　　要：目的应用流式细胞术（FCM）检测双表型急性白血病（BAL）的免疫表型。方法采用四色FCM检测23例（BAL）患者的免疫表型。结果23例BAL患者中有10例（43．4％）表达cCD3，16例（69．6％）表达cCD79，20例（87．0％）表达cMPO，14例（60．9％）表达TdT，19例（82．6％）表达CD34，20例（87．0％）表达CD117，同时表达髓系和B系抗原者13例（56．5％），均共同表达cCD79和cMPO；同时表达髓系和T系抗原者7例（30．4％），均共同表达cCD3和cMPO；同时表达T系和B系抗原者3例（13．04％），均共同表达cCD，和cCD79。结论cCD3、cCD79、cMPO为系列特异性抗原标志，对诊断及鉴别BAL具有重要意义，FCM是日前诊断BAL特异可靠的方法，在临床白血病治疗和预后方面有重要的指导意义。Objective To detect double immuno-phenotype of acute leukemia by the application of flow cytometry （FCM）. Methods Four-color FCM was used to analyse 23 cases of acute leukemia of double immune phenotype. Results Among 23 cases, cCD3 was expressed in 10 cases （43.4 %）, cCD79a in 16 cases （69.6 %）, cMPO in 20 cases （87.0 %）, TdT in 14 cases （60.9 %）, CD34 in 19 cases （82.6 %） and CD117 in 20 cases （87.0 %）. 13 cases （56.5 %） expressed both myeloid and B lymphocyte antigens, in which cCDT79a and cMPO were positively expressed, 7 cases （30.4 %） were found to express both myeloid and T lymphocyte antigens of cCD3 and cMPO 3 eases （13.04 %） were positive for both cCD3 and cCD79a. Conclusion cCD3, cCD79a9, and cMPO were lineage specific antigen markers, which could be used for diagnosis and differential double phenotype leukemias. FCM is a useful and reliable method for diagnosis of acute leukemia, and guidance of treatment and prognosis of leukemia in clinical.

关 键 词：双表型白血病 免疫表型分型 流式细胞术 

分 类 号：R733.71[医药卫生—肿瘤]