Simple diffusion delivery via brain interstitial route for the treatment of cerebral ischemia  

作　　者：HAN HongBin XIA ZuoLi CHEN He HOU Chao LI WeiBo 

机构地区：[1]Department of Radiology, Peking University Third Hospital, Beijing 100191, China [2]Department of Neurology, Affiliated Hospital, Taishan Medical College, Tai'an 271000, China [3]Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum Miinchen, German Research Center for Environmental Health (GmbH), Neuherberg 85764, Germany

出　　处：《Science China(Life Sciences)》2011年第3期235-239,共5页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos. 30972811 and 81071148);Natural Science Foundation of Beijing(Grant No. 7093137)

摘　　要：Delivering pharmacologic agents directly into the brain has been proposed as a means of bypassing the blood brain barrier.However,despite 16 years of research on a number of central nervous system disorders,an effective treatment using this strategy has only been observed in the brain tumor glioblastoma multiforme.Within this study we propose a novel system for delivering drugs into the brain named the simple diffusion (SDD) system.To validate this technique,rats were subjected to a single intracranial (at the caudate nucleus),or intraperitoneal injection,of the compound citicoline,followed two hours later by a permanent middle cerebral artery occlusion (pMCAO).Results showed that 12 h after pMCAO,with 0.0025 g kg-1 citicoline,an infarct volume 1/6 the size of the intraperitoneal group was achieved with a dose 1/800 of that required for the intraperitoneal group.These results suggest that given the appropriate injection point,through SDD a pharmacologically effective concentration of citicoline can be administered.Delivering pharmacologic agents directly into the brain has been proposed as a means of bypassing the blood brain barrier.However,despite 16 years of research on a number of central nervous system disorders,an effective treatment using this strategy has only been observed in the brain tumor glioblastoma multiforme.Within this study we propose a novel system for delivering drugs into the brain named the simple diffusion （SDD） system.To validate this technique,rats were subjected to a single intracranial （at the caudate nucleus）,or intraperitoneal injection,of the compound citicoline,followed two hours later by a permanent middle cerebral artery occlusion （pMCAO）.Results showed that 12 h after pMCAO,with 0.0025 g kg^-1 citicoline,an infarct volume 1/6 the size of the intraperitoneal group was achieved with a dose 1/800 of that required for the intraperitoneal group.These results suggest that given the appropriate injection point,through SDD a pharmacologically effective concentration of citicoline can be administered.

关 键 词：permanent middle cerebral artery occlusion animal model brain ischemic injury citicoline therapy MRI NEUROPROTECTION 

分 类 号：Q255[生物学—细胞生物学] S858.23[农业科学—临床兽医学]