Temperature-sensitive molecularly imprinted microgels with esterase activity  

作　　者：WANG HongFei YANG Hao ZHANG LiMing 

机构地区：[1]Key Laboratory for Designed Synthesis and Application of Polymer Materials,School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China [2]Key Laboratory for Polymer Composite and Functional Materials, Ministry of Education,School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China

出　　处：《Science China Chemistry》2011年第3期515-520,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21074152,20874116,20676155 and J0730420);the Natural Science Foundation of Guangdong Province in China(8151027501000004 and 9151027501000105);the Doctoral Research Program of Ministry of Education Ministry of China(20090171110023)

摘　　要：Temperature-sensitive molecularly imprinted microgels(MIGs)exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate(PAD-His)as the functional macromonomer and p-nitrophenyl phosphate(NPP)as the stable transition state analogue(TSA)as well as Co2+as the coordination center.The catalytic activity of MIGs was greatly influenced by the amount of the template,and could be modulated by temperature.The hydrolysis kinetics of p-nitrophenyl acetate(NPA)in the presence of MIGs could be described by the Michaelis-Menten equation.The MichaelisMenten constant and maximum velocity were found to be 2.2×105mol/L and 2.04×10 -8mol/h,respectively.In addition,the MIGs were found to have a high catalytic selectivity to NPA.Temperature-sensitive molecularly imprinted microgels（MIGs）exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate（PAD-His）as the functional macromonomer and p-nitrophenyl phosphate（NPP）as the stable transition state analogue（TSA）as well as Co2＋as the coordination center.The catalytic activity of MIGs was greatly influenced by the amount of the template,and could be modulated by temperature.The hydrolysis kinetics of p-nitrophenyl acetate（NPA）in the presence of MIGs could be described by the Michaelis-Menten equation.The MichaelisMenten constant and maximum velocity were found to be 2.2×105mol/L and 2.04×10 -8mol/h,respectively.In addition,the MIGs were found to have a high catalytic selectivity to NPA.

关 键 词：molecular imprinting microgels ESTERASE temperature sensitivity DEXTRAN 

分 类 号：O641.3[理学—物理化学] TQ630.6[理学—化学]