微波辅助提取积雪草总黄酮的工艺研究  

Microwave-assisted Extraction of Total Flavonoids from Centella asiatica

作　　者：程晓荣[1] 赵晨晨[1] 承伟[1] 

机构地区：[1]辽宁医学院药学院,辽宁锦州121000

出　　处：《安徽农业科学》2011年第3期1354-1355,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]在微波辅助条件下寻找提取积雪草总黄酮的优化条件。[方法]选取单因素试验和正交试验,以微波功率、微波辅助提取时间、乙醇浓度、固液比为因素,每个因素3个水平,选择L9(34)正交表,用紫外分光光度法测定的总黄酮含量作为评价指标。[结果]最佳提取条件为微波功率406 W,微波时间90 s,乙醇浓度70%,固液比1∶20,此时总黄酮含量为5.342%。[结论]该试验提取工艺操作简单、步骤简洁、方法稳定可行。[Objective] The research aimed to study the optimum condition of extraction for the total flavonoids in Centella asiatica by the microwave.[Method] The single factor test and the orthogonal test were adopted.The factors were microwave power,microwave time,ethanol concentration and solid/liquid ratio.L9（34） orthogonal test was selected to determine the content of the total flavonoids by ultraviolet spectrophotometer.[Result] The optimum condition was as follows： microwave power 406 W,microwave time 90 s,ethanol concentration 70%,solid/liquid ratio 1：20.The flavonoids content reached 5.342%.[Conclusion] Optimized extraction technology was simple,feasible and stable.

关 键 词：积雪草 总黄酮 微波辅助提取 正交试验 紫外分光光度法 

分 类 号：S37[农业科学—农产品加工]