中国金顶侧耳菌株遗传多态性的ISSR分析  

Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

作　　者：张秋胜[1] 徐丙莲[2] 刘林德[1] 程显好[1] 高兴喜[1] 史亚丽[3] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]鲁东大学数学与信息学院,山东烟台264025 [3]鲁东大学体育学院,山东烟台264025

出　　处：《食品科学》2011年第5期172-175,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2008BADA1B04);鲁东大学人才基金项目(LY20063303);鲁东大学创新团队建设项目;鲁东大学学科建设经费项目

摘　　要：金顶侧耳是重要的食药用真菌。本实验收集全国各地18种金顶侧耳菌株,通过ISSR分子标记技术分析金顶侧耳菌株种内遗传多态性,11种引物扩增出102个条带,其中78个条带具有多态性,多态率为76.4%。结果表明,金顶侧耳菌株内具有丰富的遗传多样性,遗传相似系数在0.50～0.96。采用平均分类法UPGMA分析表明,在遗传相似系数为0.62时,18种菌株分为两大类。当遗传相似系数增大到0.75时,它们分为7类。16号菌株具有独立的遗传体系。Pleurotus cituinopileatus is an important kind of edible medicinal fungi.Totally 18 kinds of Pleurotus cituinopileatus strains were collected from some markets in China.Inter-simple sequence repeat（ISSR） method was used to analyze genetic polymorphism of Pleurotus cituinopileatus strains.A total of 102 DNA fragments were amplified by using eleven ISSR primers,and 78 of these fragments were polymorphic with polymorphism rate of 76.4%.Meanwhile,high-level genetic polymorphism was observed in Pleurotus cituinopileatus and the genetic similarity coefficients（GSC） were between 0.50 and 0.96.These 18 strains were divided into 2 groups at GSC of 0.62 and 7 groups at GSC of 0.75 according to the cluster analysis of UPGMA method.Strain 16 had an independent genetic system.These results will provide a scientific fundament to accelerate genetic breeding and identification of cultivated strains of Pleurotus cituinopileatus.

关 键 词：金顶侧耳 简短重复序列 多态性 亲缘关系 

分 类 号：S646[农业科学—蔬菜学]