鸡CAPN1基因多态性及其与肉质性状的关联分析  

Polymorphism of CAPN1 Gene and Its Correlation with Meat Quality Traits in Shouguang Chicken

作　　者：周国利[1] 郭彦[1] 方翔[1] 吴玉厚[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《食品科学》2011年第5期207-210,共4页Food Science

基　　金：山东省自然科学基金项目(Y2007D33)

摘　　要：采用聚合酶链式反应-单链构象多态(PCR-SSCP)方法对147只寿光鸡CAPN1基因第4外显子进行多态性检测,分析其与部分肉质性状的关系。在CAPN1基因第4外显子上共检测到3种基因型AA、AB和BB,它们的基因型频率分别为0.286、0.510和0.204。方差分析结果表明:在寿光鸡群体中,BB基因型个体的胸肌pHu值显著高于AA基因型个体(P<0.05);AA基因型个体的肌内脂肪含量显著高于BB基因型个体(P<0.05)。Polymerase chain reaction-single strand conformation polymorphism（PCR-SSCP） method was used to determine the polymorphism of exon 4 of CAPN1 gene in 147 Shouguang chicken,and analyze the correlation between CAPN1 gene and meat quality traits in Shouguang chicken.Three genotypes were identified as AA,AB and BB with the genotype frequencies of 0.286,0.510 and 0.204,respectively.Statistical analysis indicated that type BB Shouguang chicken had significantly higher breast muscle pHu value than type AA（P〈0.05）.However,type AA Shouguang chicken had significantly higher content of intramuscular fat than type BB（P〈0.05）.

关 键 词：寿光鸡 聚合酶链式反应-单链构象多态(PCR-SSCP) CAPN1基因 肉质性状 

分 类 号：S831.2[农业科学—畜牧学] R393.11[农业科学—畜牧兽医]