沙棘茶水溶性多糖对衰老模型小鼠抗氧化能力的影响  

Antioxidant Effect of Water Soluble Polysaccharides from Commercially Available Hippophae rhamnoides Leaf Tea in Aging Model Mice

作　　者：李芳亮[1] 王锐[1] 杜秋野[1] 孙伟[1] 韩宝亮[1] 

机构地区：[1]辽宁工程技术大学理学院,辽宁阜新123000

出　　处：《食品科学》2011年第5期284-286,共3页Food Science

基　　金：辽宁工程技术大学校优秀青年科学研究基金项目(07-127)

摘　　要：为了研究沙棘茶水溶性多糖(WPHT)对衰老模型小鼠体内抗氧化能力的影响。本实验通过连续颈部皮下注射D-半乳糖(0.5g/(kg.d))建立小鼠亚急性衰老模型,第11天开始同时灌胃给予沙棘茶水溶性多糖10、50、100mg/(kg.d),40d后检测各实验组血浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量和肝脏匀浆液中SOD活性、过氧化氢酶(CAT)活性和MDA的含量。结果表明,50mg/(kg.d)和100mg/(kg.d)的WPHT可以提高血浆中SOD活性和肝脏匀浆液中的SOD和CAT活性,降低血浆和肝脏匀浆液中的MDA含量,与衰老模型组相比差异显著(P<0.05)。说明WPHT可以提高D-半乳糖致亚急性衰老小鼠的抗氧化能力。The purpose of the present work was to study the antioxidant effect of water soluble polysaccharides from Hippophae rhamnoides leaf tea（WPHT）,a commercially available product processed from the leaves of the plant,in subacute aging model mice induced by subcutaneous injection of D-galactose.Fifty mice were divided into 5 groups of 10 ones each： blank control group,aging model group,low-dose 10 mg/（kg·d） WPHT group,middle-dose 50 mg/（kg·d） WPHT group and high-dose 100 mg/（kg·d） WPHT group.The aging model group and the low-,middle-and high-dose WPHT groups were all injected with D-galactose（0.5 g/（kg·d）） subcutaneously for 40 consecutive days,and normal saline instead of D-galactose was given to the blank control group.Intragastric administration of WPHT began after 10 days of subcutaneous injection of D-galactose,was carried out once a day and lasted 30 days.All the mice were fasted for 12 hours after the last intragastric administration of WPHT,and were then scarified to determine the activities of plasma superoxide dismutase（SOD）,the content of malondialdehyde（MDA） in plasma,and the activities of SOD,catalase（CAT） and MDA content in liver homogenate.The results revealed that WPHT administration at 50 mg/（kg·d） and 100 mg/（kg·d） could elevate the activities of plasma SOD and liver homogenate SOD and CAT and decrease the contents of plasma MDA and liver homogenate MDA,with a significant difference from the model group（P 0.05）.Therefore,WPHT has antioxidant potential in vivo.

关 键 词：沙棘茶 多糖 抗氧化 超氧化物歧化酶 

分 类 号：Q946.91[生物学—植物学] R285.5[医药卫生—中药学]